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HSP60 Antibody 26 Publications

Rabbit Polyclonal| Catalog number: 15282-1-AP

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Con: 47 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

HSP60 Antibody WB HeLa cells 15282-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 15282-1-AP(HSP60 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours

HSP60 Antibody IHC human lung cancer tissue 15282-1-AP

Immunohistochemical of paraffin-embedded human lung cancer using 15282-1-AP(HSP60 antibody) at dilution of 1:100 (under 10x lens)

HSP60 Antibody IHC human lung cancer tissue 15282-1-AP

Immunohistochemical of paraffin-embedded human lung cancer using 15282-1-AP(HSP60 antibody) at dilution of 1:100 (under 40x lens)

HSP60 Antibody IP HEK-293 cells 15282-1-AP

IP Result of anti-HSP60 (IP:15282-1-AP, 4ug; Detection:15282-1-AP 1:4000) with HEK-293 cells lysate 3680ug.

HSP60 Antibody IF HepG2 cells 15282-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 15282-1-AP(HSP60 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, chicken, rice
Positive WB detected in:
HeLa cells, HepG2 cells, NIH/3T3 cells, rat heart tissue
Positive IP detected in:
HEK-293 cells
Positive IHC detected in:
human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:3000-1:10000
IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
HSP60 fusion protein Ag7401
Full name:
heat shock 60kDa protein 1 (chaperonin)
Calculated molecular weight:
61 kDa
Observed molecular weight:
60 kDa
GenBank accession number:
BC003030
Gene ID (NCBI):
3329
Gene symbol
HSPD1
Synonyms
HSP 60, HSP60, HSP65, HSPD1

Background

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)


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