|Positive WB detected in||HEK-293 cells, HepG2 cells, NIH/3T3 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
15282-1-AP targets HSP60 in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||chicken, fish, human, mouse, pig, rat, rice|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HSP60 fusion protein Ag7401|
|Full Name||heat shock 60kDa protein 1 (chaperonin)|
|Calculated molecular weight||61 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC003030|
|Gene ID (NCBI)||3329|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)
|Product Specific Protocols|
|WB protocol for HSP60 antibody 15282-1-AP||Download protocol|
|IHC protocol for HSP60 antibody 15282-1-AP||Download protocol|
|IF protocol for HSP60 antibody 15282-1-AP||Download protocol|
|IP protocol for HSP60 antibody 15282-1-AP||Download protocol|
|FC protocol for HSP60 antibody 15282-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.
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Fetal bovine serum inhibits neomycin-induced apoptosis of hair cell-like HEI-OC-1 cells by maintaining mitochondrial function.
Protective effects of ghrelin on brain mitochondria after cardiac arrest and resuscitation.
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Arsenic and/or copper caused inflammatory response via activation of inducible nitric oxide synthase pathway and triggered heat shock protein responses in testis tissues of chicken.
Impacts of simultaneous exposure to arsenic (III) and copper (II) on inflammatory response, immune homeostasis, and heat shock response in chicken thymus.
Extensive skeletal muscle cell mitochondriopathy distinguishes critical limb ischemia patients from claudicants.