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HSP60 Polyclonal antibody

HSP60 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15282-1-AP

Synonyms

HSP 60, HSP60, HSP65, HSPD1



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, NIH/3T3 cells, HeLa, mouse heart, rat heart
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15282-1-AP targets HSP60 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, chicken, fish, pig, rice
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP60 fusion protein Ag7401
Full Name heat shock 60kDa protein 1 (chaperonin)
Calculated molecular weight 61 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC003030
Gene symbol HSPD1
Gene ID (NCBI) 3329
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

Protocols

Product Specific Protocols
WB protocol for HSP60 antibody 15282-1-APDownload protocol
IHC protocol for HSP60 antibody 15282-1-APDownload protocol
IF protocol for HSP60 antibody 15282-1-APDownload protocol
IP protocol for HSP60 antibody 15282-1-APDownload protocol
FC protocol for HSP60 antibody 15282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.

Authors - Xiaorong Zhang
humanWB

Nat Commun

Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.

Authors - Wenjun Wang
mouseWB,IF

Nat Commun

Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells.

Authors - Gustavo Della-Flora Nunes
humanWB

Nat Commun

EGFL9 promotes breast cancer metastasis by inducing cMET activation and metabolic reprogramming.

Authors - Fanyan Meng
mouseWB,IF

J Clin Invest

ATM inhibition enhances cancer immunotherapy by promoting mtDNA leakage/cGAS-STING activation.

Authors - Mengjie Hu
mouseIF

Theranostics

AdMSC-derived exosomes alleviate acute lung injury via transferring mitochondrial component to improve homeostasis of alveolar macrophages.

Authors - Liangjun Xia