HSP10 Polyclonal antibody

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  WB analysis using 16512-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16512-1-AP (HSP10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 16512-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16512-1-AP (HSP10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16512-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16512-1-AP (HSP10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse adrenal gland using 16512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 16512-1-AP (HSP10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse adrenal gland using 16512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 16512-1-AP (HSP10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HSP10 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16512-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

HSPE1, 10 kDa chaperonin, CPN10, Early-pregnancy factor, Heat shock protein family E member 1

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2
• Positive IHC detected in: human lung cancer tissue, mouse adrenal gland tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16512-1-AP targets HSP10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSP10 fusion protein Ag9732
• Full Name: heat shock 10kDa protein 1 (chaperonin 10)
• Calculated Molecular Weight: 102 aa, 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC023518
• Gene Symbol: HSP10
• Gene ID (NCBI): 3336
• RRID: AB_3085493
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61604
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.