Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, A549 cells, U2OS cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67168-1-Ig targets HUS1 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28527 Product name: Recombinant human HUS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-280 aa of BC007013 Sequence: MKFRAKIVDGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS Predict reactive species |
| Full Name | HUS1 checkpoint homolog (S. pombe) |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC007013 |
| Gene Symbol | HUS1 |
| Gene ID (NCBI) | 3364 |
| RRID | AB_2882464 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O60921 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Hus1 belongs to the Rad family, form a heterotrimeric clamp with Rad1and Rad9 that acts as a putative sensor for DNA damage. This complex plays an apical role in the DNA damage response, loss of a functional complex affects many downstream checkpoint pathways [PMID: 11340080]. It is also a key complex involved in the activation of the ATR pathway. 9-1-1 forms a ring-shaped heterotrimer, resembling the homotrimeric sliding clamp, proliferating cell nuclear antigen (PCNA) [PMID: 19535328].
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HUS1 antibody 67168-1-Ig | Download protocol |
| WB protocol for HUS1 antibody 67168-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
One customer rated this antibody 4 out of 5 stars for immunofluorescence on HeLa cells. They used a 1:300 dilution with 5% FBS blocking and overnight primary antibody incubation. The protocol involved pre-extracting cells for 5 minutes before fixation with 4% PFA for 15 minutes. The resulting image clearly showed chromatin-bound HUS1 in wild-type HeLa cells, demonstrating good specificity for nuclear localization.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hasan (Verified Customer) (05-17-2023) | Image showing chromatin-bound HUS1 in HeLa WT cells, pre-extracted for 5 min before fixation with 4% PFA for 15 min. Blocking with 5% FBS and primary antibody overnight.
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