HUS1 Monoclonal antibody

HUS1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E8E9

Cat no : 67168-1-Ig

Synonyms

Checkpoint protein HUS1, hHUS1, HUS1



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, A549 cells, U2OS cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

67168-1-Ig targets HUS1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HUS1 fusion protein Ag28527
Full Name HUS1 checkpoint homolog (S. pombe)
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC007013
Gene symbol HUS1
Gene ID (NCBI) 3364
RRIDAB_2882464
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hus1 belongs to the Rad family, form a heterotrimeric clamp with Rad1and Rad9 that acts as a putative sensor for DNA damage. This complex plays an apical role in the DNA damage response, loss of a functional complex affects many downstream checkpoint pathways [PMID: 11340080]. It is also a key complex involved in the activation of the ATR pathway. 9-1-1 forms a ring-shaped heterotrimer, resembling the homotrimeric sliding clamp, proliferating cell nuclear antigen (PCNA) [PMID: 19535328].

Protocols

Product Specific Protocols
WB protocol for HUS1 antibody 67168-1-IgDownload protocol
IHC protocol for HUS1 antibody 67168-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hasan (Verified Customer) (05-17-2023)

Image showing chromatin-bound HUS1 in HeLa WT cells, pre-extracted for 5 min before fixation with 4% PFA for 15 min. Blocking with 5% FBS and primary antibody overnight.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: HeLa cells
HUS1 Antibody Immunofluorescence validation (1:300 dilution) in HeLa cells (Cat no:67168-1-Ig)