HUS1 Monoclonal antibody, PBS Only

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  WB analysis using 67168-1-Ig (same clone as 67168-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67168-1-Ig (HUS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67168-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67168-1-Ig (same clone as 67168-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67168-1-Ig (HUS1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67168-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67168-1-Ig (same clone as 67168-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67168-1-Ig (HUS1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67168-1-PBS in a different storage buffer formulation.

HUS1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67168-1-PBS

Clone No.1E8E9

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Synonyms

Checkpoint protein HUS1, hHUS1, HUS1

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Product Information

67168-1-PBS targets HUS1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28527

  Product name: Recombinant human HUS1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-280 aa of BC007013

  Sequence: MKFRAKIVDGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS

• Full Name: HUS1 checkpoint homolog (S. pombe)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC007013
• Gene Symbol: HUS1
• Gene ID (NCBI): 3364
• RRID: AB_2882464
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O60921
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Hus1 belongs to the Rad family, form a heterotrimeric clamp with Rad1and Rad9 that acts as a putative sensor for DNA damage. This complex plays an apical role in the DNA damage response, loss of a functional complex affects many downstream checkpoint pathways [PMID: 11340080]. It is also a key complex involved in the activation of the ATR pathway. 9-1-1 forms a ring-shaped heterotrimer, resembling the homotrimeric sliding clamp, proliferating cell nuclear antigen (PCNA) [PMID: 19535328].