CoraLite®594-conjugated Heavy chain of Rabbit IgG Monoclonal antibody

Heavy chain of Rabbit IgG Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

rabbit

Applications

IHC, IF

Conjugate

CoraLite®594 Fluorescent Dye

Publications

4

CloneNo.

1C9A5

Cat no : CL594-66467

Synonyms

Rabbit IgG

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66467

  Immunofluorescent analysis of (-20°C Acetone) fixed HeLa cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:100 and CL594-66467 (CL594-Mouse anti-Rabbit IgG heavy chain) as secondary antibody with dilution 1:400. .

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Tested Applications

• Positive IF detected in: HeLa cells

For other applications, we recommend the unconjugated version of this antibody, 66467-1-Ig

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

CL594-66467 targets Heavy chain of Rabbit IgG in IHC, IF applications and shows reactivity with rabbit samples.

• Tested Reactivity: rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Protein
• Full Name: Heavy chain of Rabbit IgG
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 593 nm/614 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

This antibody specifically recognizes the heavy chain of Rabbit IgG. It can't detect the The heavy chain of other species. This antibody can be used as the secondary antibody which is specific to the heavy chain of Rabbit IgG. This anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Publications

Species	Application	Title
	IHC	Front Med (Lausanne); Teasaponin Ameliorates Murine Colitis by Regulating Gut Microbiota and Suppressing the Immune System Response.; Authors - Huan Yang
	IF	Mol Neurobiol; Maternal Subclinical Hypothyroidism in Rats Impairs Spatial Learning and Memory in Offspring by Disrupting Balance of the TrkA/p75NTR Signal Pathway.; Authors - Fan Zhang
		FASEB J; Senolytic therapy ameliorates renal fibrosis postacute kidney injury by alleviating renal senescence.; Authors - Caizhen Li
		Nucleic Acids Res; MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis.; Authors - Yongliang Shang