Filter: AllWBIPIHC

WB analysis using 55047-1-AP (same clone as 55047-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 55047-1-AP (HuC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55047-1-PBS in a different storage buffer formulation.
IP experiment of Neuro-2a using 55047-1-AP (same clone as 55047-1-PBS)
IP result of anti-HuC (IP:55047-1-AP, 4ug; Detection:55047-1-AP 1:5000) with Neuro-2a cells lysate 1440 ug. This data was developed using the same antibody clone with 55047-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 55047-1-AP (same clone as 55047-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55047-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 55047-1-AP (same clone as 55047-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55047-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 55047-1-AP (same clone as 55047-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55047-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 55047-1-AP (same clone as 55047-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55047-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

HuC Polyclonal antibody, PBS Only

HuC Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 55047-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

ELAVL 3, ELAVL3, ELAV-like protein 3, Hu antigen C, Hu-antigen C

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

55047-1-PBS targets HuC in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C)
Calculated Molecular Weight	40 kDa
Observed Molecular Weight	36-38 kDa
GenBank Accession Number	NM_001420
Gene Symbol	HuC
Gene ID (NCBI)	1995
RRID	AB_10859256
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14576
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

HUC , belongs to the RRM elav family. It binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. ELAVL3 may also bind poly-A tracts via RRM 3. It is involved in neuronal differentiation and maintenance. The antibody has no cross reaction to other ELAVL members.