HuC Polyclonal antibody

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  WB analysis using 55047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55047-1-AP (HuC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of Neuro-2a using 55047-1-AP

  IP result of anti-HuC (IP:55047-1-AP, 4ug; Detection:55047-1-AP 1:5000) with Neuro-2a cells lysate 1440 ug.

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  IHC staining of human brain using 55047-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 55047-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 55047-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 55047-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 10x lens).

• Featured Product
• KD/KO Validated

HuC Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 55047-1-AP

Publications(9)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, RIP, ELISA

Synonyms

ELAVL 3, ELAVL3, ELAV-like protein 3, Hu antigen C, Hu-antigen C

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Tested Applications

• Positive WB detected in: mouse brain tissue, Neuro-2a cells
• Positive IP detected in: Neuro-2a cells
• Positive IHC detected in: human lung cancer tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• RIP: See 1 publications below

Product Information

55047-1-AP targets HuC in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C)
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 36-38 kDa
• GenBank Accession Number: NM_001420
• Gene Symbol: HuC
• Gene ID (NCBI): 1995
• RRID: AB_10859256
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14576
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HUC , belongs to the RRM elav family. It binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. ELAVL3 may also bind poly-A tracts via RRM 3. It is involved in neuronal differentiation and maintenance. The antibody has no cross reaction to other ELAVL members.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Acta Neuropathol; Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis.; Authors - Sandra Diaz-Garcia
human	WB,IF,RIP	Nucleic Acids Res; HuB and HuD repress telomerase activity by dissociating HuR from TERC.; Authors - Xiaolei Cheng
zebrafish	IF	Invest Ophthalmol Vis Sci; Deficiency of Acetyltransferase nat10 in Zebrafish Causes Developmental Defects in the Visual Function; Authors - Hou-Zhi Yang
human	WB	Am J Pathol; Anaplastic lymphoma kinase (ALK) overexpression is associated with aggressive phenotypic characteristics of ovarian high-grade serous carcinoma.; Authors - Toshihide Matsumoto
human	IF	Cell Stem Cell; KCNJ2 inhibition mitigates mechanical injury in a human brain organoid model of traumatic brain injury; Authors - Jesse D Lai
human	WB	iScience; Multivalent GU-rich oligonucleotides sequester TDP-43 in the nucleus by inducing high molecular weight RNP complexes; Authors - Xi Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Raquel (Verified Customer) (06-21-2024)

Immunofluorescence analysis in cryostat sections of 4% PFA fixed human brain organoid derived from iPSCs using 55047-1-AP . HuC antibody at dilution of 1:50 (under 20x lens). (Green: HuC; Blue: DAPI)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Brain organoid made with human iPSCs

HuC Antibody Immunofluorescence validation (1:50 dilution) in Brain organoid made with human iPSCs (Cat no:55047-1-AP)

FH

Etienne (Verified Customer) (12-19-2022)

Did not work. Virtually only aspecific binding in human brain organoids

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/20, 1/50, 1/200
• Cell Tissue Type: Human Brain Organoids

HuC Antibody Immunofluorescence validation (1/20, 1/50, 1/200 dilution) in Human Brain Organoids (Cat no:55047-1-AP)

FH

Alessandro (Verified Customer) (11-07-2022)

no unspecific staining, great outcome

• Applications: Immunofluorescence

FH

David (Verified Customer) (09-01-2022)

Works good!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: Human brain

HuC Antibody Immunofluorescence validation (1:150 dilution) in Human brain (Cat no:55047-1-AP)