Tested Applications
| Positive WB detected in | mouse brain tissue, Neuro-2a cells |
| Positive IP detected in | Neuro-2a cells |
| Positive IHC detected in | human lung cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55047-1-AP targets HuC in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 36-38 kDa |
| GenBank Accession Number | NM_001420 |
| Gene Symbol | HuC |
| Gene ID (NCBI) | 1995 |
| RRID | AB_10859256 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14576 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HUC , belongs to the RRM elav family. It binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. ELAVL3 may also bind poly-A tracts via RRM 3. It is involved in neuronal differentiation and maintenance. The antibody has no cross reaction to other ELAVL members.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HuC antibody 55047-1-AP | Download protocol |
| IP protocol for HuC antibody 55047-1-AP | Download protocol |
| WB protocol for HuC antibody 55047-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HuC (ELAVL3) polyclonal antibody (Cat# 55047-1-AP) has 13 citations published in journals including Cell Stem Cell, Nucleic Acids Research, Acta Neuropathologica, EMBO Journal, Communications Biology, iScience, American Journal of Pathology, Frontiers in Cellular Neuroscience, and others. Research fields span RNA biology (splicing, polyadenylation), neurodegeneration (ALS), neuronal development, oncology (neuroblastoma, ovarian carcinoma), developmental biology, and gastroenterology.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell KCNJ2 inhibition mitigates mechanical injury in a human brain organoid model of traumatic brain injury | ||
Acta Neuropathol Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis. | ||
Int J Biol Macromol Multi-omics revealed that ELAVL3 regulates MYCN in neuroblastoma via immunogenic cell death: Risk stratification and experimental research | ||
EMBO J Nuclear paxillin functions as a molecular switch for alternative splicing in neurons during a critical period of brain development. | ||
Commun Biol HuB serves as the central hub for connecting HuR-related mRNAs with translation machinery in inflammation. |
Reviews
What customers say
Most reviewers report positive results with this antibody for immunofluorescence. Three out of four users rated it 5 stars, noting clean staining with minimal background in human brain tissue and iPSC-derived brain organoids at dilutions ranging from 1:50 to 1:150. One reviewer gave a lower rating, reporting mostly non-specific binding in human brain organoids despite testing multiple dilutions (1:20, 1:50, 1:200). Overall, the antibody is well-received for IF applications on neural tissues, though individual results may vary depending on sample preparation and conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Raquel (Verified Customer) (06-21-2024) | Immunofluorescence analysis in cryostat sections of 4% PFA fixed human brain organoid derived from iPSCs using 55047-1-AP . HuC antibody at dilution of 1:50 (under 20x lens). (Green: HuC; Blue: DAPI)
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Etienne (Verified Customer) (12-19-2022) | Did not work. Virtually only aspecific binding in human brain organoids
 |
Alessandro (Verified Customer) (11-07-2022) | no unspecific staining, great outcome
|
David (Verified Customer) (09-01-2022) | Works good!
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