HuR/ELAVL1 Polyclonal antibody

• 

  WB analysis using 11910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11910-1-AP (HuR/ELAVL1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11910-1-AP

  WB result of HuR/ELAVL1 antibody (11910-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HuR transfected HEK-293 cells.

• 

  IP experiment of HEK-293 using 11910-1-AP

  IP result of anti-HuR/ELAVL1 (IP:11910-1-AP, 4ug; Detection:11910-1-AP 1:20000) with HEK-293 cells lysate 1400 ug.

• 

  IHC staining of human breast cancer using 11910-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11910-1-AP (HuR/ELAVL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11910-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11910-1-AP (HuR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11910-1-AP (HuR/ELAVL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11910-1-AP (HuR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 11910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HuR/ELAVL1 antibody (11910-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of MCF-7 using 11910-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human HuR/ELAVL1 (11910-1-AP) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

HuR/ELAVL1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11910-1-AP

Publications(104)

Reviews (6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, CLIP

Synonyms

ELAVL1, Hua, Hu antigen R, ELAV-like protein 1, ELAVL 1

---

Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 23 publications below
• WB: See 78 publications below
• IHC: See 10 publications below
• IF: See 25 publications below
• IP: See 3 publications below
• CoIP: See 5 publications below
• ChIP: See 1 publications below
• RIP: See 13 publications below

Product Information

11910-1-AP targets HuR/ELAVL1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, CLIP applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2249

  Product name: Recombinant human HuR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-326 aa of BC003376

  Sequence: MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK

• Full Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
• Calculated Molecular Weight: 326 aa, 36 kDa
• Observed Molecular Weight: 30-37 kDa
• GenBank Accession Number: BC003376
• Gene Symbol: HuR/ELAVL1
• Gene ID (NCBI): 1994
• RRID: AB_11182183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15717
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Cancer; SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers; Authors - Lei Shi
human	WB	Nat Protoc; Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture.; Authors - Joel I Perez-Perri
human	IF	Acta Neuropathol; Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis.; Authors - Sandra Diaz-Garcia
	WB,IF	Adv Sci (Weinh); Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia; Authors - Ningjing Ou
mouse	WB	Mol Cell; Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.; Authors - Jing Wen
human	WB	Autophagy; m6A reader YTHDC1 modulates autophagy by targeting SQSTM1 in diabetic skin; Authors - Diefei Liang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Adrianna (Verified Customer) (10-24-2022)

This antibody works very well - strong detection without unspecific bands. I usually incubate it over night at 4 deg C.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF7

FH

PK (Verified Customer) (07-08-2022)

Very Good

• Applications: Western Blot
• Cell Tissue Type: H9C2

HuR/ELAVL1 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:11910-1-AP)

FH

S (Verified Customer) (04-19-2021)

Excellent!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: AC16

HuR/ELAVL1 Antibody Western Blot validation (1:1000 dilution) in AC16 (Cat no:11910-1-AP)

FH

S (Verified Customer) (05-18-2020)

Good Antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: AC16

HuR/ELAVL1 Antibody Western Blot validation (1:1000 dilution) in AC16 (Cat no:11910-1-AP)

FH

CHAO (Verified Customer) (01-30-2020)

Weak band for the heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

FH

Gaston (Verified Customer) (05-16-2019)

Antibody worked very well for wester blot detection and immunofluorescence in Huh7 and SH-SY5Y cells. Did not observe any signs of deteriorating detection when stored at -20 for over two years.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:250; 1:6500
• Cell Tissue Type: Huh7, SH-SY5Y, Vero

HuR/ELAVL1 Antibody Western Blot,Immunofluorescence, validation (1:250; 1:6500 dilution) in Huh7, SH-SY5Y, Vero (Cat no:11910-1-AP)