Human A1BG ELISA Kit
Cat no : KE00130
Synonyms
Alpha 1B-Glycoprotein, A1B, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein
Validation Data Gallery
Product Information
KE00130 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The A1BG ELISA kit is to be used to detect and quantify protein levels of endogenous A1BG. The assay recognizes human A1BG. An antibody specific for A1BG has been pre-coated onto the microwells. The A1BG protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for A1BG is added to detect the captured A1BG protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human A1BG ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 21.5 pg/mL |
| Range | 125-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 104% | 90%-125% |
| Human plasma | 101% | 77%-122% |
| Cell culture supernatants | 98% | 80%-111% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 257.9 | 14.7 | 5.7 |
| 2 | 20 | 517.3 | 23.3 | 4.5 |
| 3 | 20 | 2,133.3 | 107.2 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 266.1 | 21.2 | 8.0 |
| 2 | 24 | 504.5 | 35.8 | 7.1 |
| 3 | 24 | 2,072.8 | 105.0 | 5.1 |
Background Information
Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. Alpha-1B-glycoprotein contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of alpha-1B-glycoprotein. Alpha-1B-glycoprotein-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3. Alpha-1B-glycoprotein is present in normal adult plasma at an average concentration of 22 mg/dl. Elevated alpha-1B-glycoprotein levels in blood have been associated with several kinds of cancer, including non-small cell lung cancer, endometrial and cervical cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Alpha 1B-Glycoprotein, A1B, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Cancers (Basel) Alpha-1B Glycoprotein Is a Novel Hepatocyte-Derived Host Factor Associated with In Vitro Inhibition of HBV Replication and Hepatocellular Carcinoma Progression. |