Human A1BG ELISA Kit0 Publications

Catalog number: KE00130

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$599.00

Freight/Packing £00.00

Size: 09µ/gram

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Overview


Product name:
Human A1BG ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatants

Assay type:
Sandwich

Sensitivity:
21.5 pg/mL

Range:
125-4000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Human serum 104% 90%-125%
Human plasma 101% 77%-122%
Cell culture supernatants 98% 80%-111%

IntraAssay:
Samplenmean ( pg/mL)SDCV%
1 20 257.9 14.7 5.7
2 20 517.3 23.3 4.5
3 20 2,133.3 107.2 5.0

InterAssay:
Samplenmean ( pg/mL)SDCV%
1 24 266.1 21.2 8.0
2 24 504.5 35.8 7.1
3 24 2,072.8 105.0 5.1

Product overview:
KE00130 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The A1BG ELISA kit is to be used to detect and quantify protein levels of endogenous A1BG. The assay recognizes human A1BG. A monoclonal antibody specific for A1BG has been pre-coated onto the microwells. The A1BG protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for A1BG is added to detect the captured A1BG protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
A1B, A1BG, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein, DKFZp686F0970, GAB, HYST2477
Background

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. Alpha-1B-glycoprotein contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of alpha-1B-glycoprotein. Alpha-1B-glycoprotein-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3. Alpha-1B-glycoprotein is present in normal adult plasma at an average concentration of 22 mg/dl. Elevated alpha-1B-glycoprotein levels in blood have been associated with several kinds of cancer, including non-small cell lung cancer, endometrial and cervical cancer.


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