Human Adiponectin ELISA Kit

Sensitivity

24.0 pg/mL

Range

62.5-4000 pg/mL

Reactivity

Human

Cat no : KE00080

Synonyms

ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein



Product Information

KE00080 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Adiponectin ELISA kit is to be used to detect and quantify protein levels of endogenous Adiponectin. The assay recognizes human Adiponectin. An antibody specific for Adiponectin has been pre-coated onto the microwells. The Adiponectin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Adiponectin is added to detect the captured Adiponectin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human Adiponectin ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 24.0 pg/mL
Range 62.5-4000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plasma95%69%-116%
Cell culture supernatants100%81%-115%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120829.076.79.3
2201,708.0164.59.6
3202,354.9158.36.7

InterAssay

Samplenmean ( pg/mL)SDCV%
124681.758.08.5
2241,421.9138.09.7
3242,467.6156.26.3

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving insulin sensitivity and anti-inflammatory activity. The role of Adiponectin in reproduction is not yet fully understood, but the expression of Adiponectin in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane Adiponectin receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. Adiponectin May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein

Publications

SpeciesSample TypeTitle
human

J Food Biochem

Attenuation of lipid accumulation in Bel-7402 cells through ADPN/AMPKα signaling stimulated by Fructus rosae laxae extract.

Authors - Ai-Rong Zhang