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Recombinant Human B3GAT1/CD57 protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0793

Validation Data Gallery

  • Purity of Recombinant Human B3GAT1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human B3GAT1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human B3GAT1 protein Thr28-Ile334 (Accession# Q9P2W7-1) with a His tag at the N-terminus.
GeneID 27087
Accession Q9P2W7-1
PredictedSize 36.1 kDa
SDS-PAGE 38-45 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

B3GAT1, or beta-1,3-glucuronyltransferase 1, is a gene located in humans that encodes a member of the glucuronyltransferase gene family. This enzyme plays a critical role in the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1), also known as CD57 and LEU7. Research has implicated B3GAT1 in various disorders, particularly those related to the immune system and infections. For instance, the enzyme's role in synthesizing glycosaminoglycans contributes to the structural integrity of cellular surfaces, which can influence susceptibility to viral infections.

References:

1.Yasuhiko Kizuka, et al. (2021) Glycoscience Protocols. 
2.Trimarco JD, et al. (2022) Nat Commun. 3 (1):6456.