Human CD23/FCER2 ELISA Kit
Cat no : KE00107
Synonyms
CD23, BLAST 2, BLAST-2, CD23A, CLEC4J
Validation Data Gallery
Product Information
KE00107 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD23 ELISA kit is to be used to detect and quantify protein levels of endogenous CD23. The assay recognizes human CD23. An antibody specific for CD23 has been pre-coated onto the microwells. The CD23 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for CD23 is added to detect the captured CD23 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD23/FCER2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine |
| Assay type | Sandwich |
| Sensitivity | 2.2 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2208 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 106% | 96%-122% |
| Human plasma | 101% | 87%-119% |
| Cell culture supernatants | 104% | 82%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 879.9 | 41.2 | 4.7 |
| 2 | 20 | 216.9 | 9.9 | 4.6 |
| 3 | 20 | 49.9 | 4.7 | 9.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 849.4 | 72.4 | 8.5 |
| 2 | 24 | 209.6 | 18.5 | 8.8 |
| 3 | 24 | 48.2 | 4.1 | 8.6 |
Background Information
CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells. CD23 can be cleaved into soluble fragment (soluble CD23, sCD23) of various sizes displaying pleiotropic biological activities. Increased plasma levels of sCD23 have been reported in allergic disorders, acute viral diseases, lepromatous leprosy, B-CLL, and inflammatory diseases like RA, SLE and sarcoidosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD23, BLAST 2, BLAST-2, CD23A, CLEC4J |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.