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Recombinant Human CD3 gamma protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2147

Validation Data Gallery

  • Purity of Recombinant Human CD3 gamma was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD3 gamma was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CD3 gamma protein Gln23-Ser116 (Accession# P09693) with a rabbit IgG Fc tag at the C-terminus.
GeneID 917
Accession P09693
PredictedSize 37.0 kDa
SDS-PAGE 40-55 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 gamma/CD3G is a single-pass type I membrane protein which consists of an extracellular domain of 94 amino acids, a transmembrane region, and a cytoplasmic domain of 45 amino acids. In addition to this role of signal transduction in T-cell activation, CD3 gamma plays an essential role in the dynamic regulation of TCR expression at the cell surface.

References:

1. J Dietrich, et al. (1994) EMBO J. 13(9):2156-66.
2. J Dietrich, et al. (1996) J Cell Biol. 132(3):299-310. 
3. E A Padlan, et al. (1997) Curr Biol. 7(1):R17-20. 
4. Clifford S Guy, et al. (2009) Immunol Rev. 232(1):7-21.