Human F12 ELISA Kit
Cat no : KE00034
Synonyms
Coagulation factor XII, F12, HAE3, HAEX, HAF, Hageman factor
Validation Data Gallery
Product Information
KE00034 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The F12 ELISA kit is to be used to detect and quantify protein levels of endogenous F12. The assay recognizes human F12. An antibody specific for F12 has been pre-coated onto the microwells. The F12 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for F12 is added to detect the captured F12 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human F12 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.5-32 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2161 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 94% | 73%-124% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 12.18 | 0.77 | 6.40 |
| 2 | 20 | 2.90 | 0.19 | 6.70 |
| 3 | 20 | 0.55 | 0.03 | 6.00 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 12.29 | 1.26 | 10.30 |
| 2 | 24 | 2.95 | 0.19 | 6.30 |
| 3 | 24 | 0.61 | 0.03 | 5.50 |
Background Information
Factor XII (FXII, Hageman factor) is a 80kD, single chain glycoprotein that circulates in blood as an inactive zymogen. FXII plays an important role in blood coagulation, fibrinolysis, and kinin generation. When plasma comes into contact with negatively charged surfaces such as glass, kaolin, or dextran sulfate, FXII is bound to the surface and undergoes limited proteolysis by plasma kallikrein, resulting in two active enzyme forms, ɑ-FXIIa and β-FXIIa. Hereditary deficiencies in FXII are not associated with spontaneous or excessive bleeding. FXII inhibition also have protective role against arterial thrombosis and stroke, suggesting that FXII may be a target for antithrombotic therapy. This kit is used to quantify FXII in vivo.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Coagulation factor XII, F12, HAE3, HAEX, HAF, Hageman factor |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.