Human GMFB ELISA Kit
Cat no : KE00001
Synonyms
Glia maturation factor beta, glia maturation factor, beta, GMF, GMF beta, GMFB
Validation Data Gallery
Product Information
KE00001 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GMFB ELISA kit is to be used to detect and quantify protein levels of endogenous GMFB. The assay recognizes human GMFB. An antibody specific for GMFB has been pre-coated onto the microwells. The GMFB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for GMFB is added to detect the captured GMFB protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GMFB ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 23.8 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2764 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plamsa | 87% | 75%-102% |
| Cell culture supernatants | 91% | 83%-99% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 6,361.0 | 413.5 | 6.5 |
| 2 | 20 | 2,641.6 | 143.3 | 5.4 |
| 3 | 20 | 1,467.3 | 102.0 | 7.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 5,734.9 | 753.2 | 13.1 |
| 2 | 24 | 2,553.0 | 164.0 | 6.4 |
| 3 | 24 | 1,509.7 | 83.0 | 5.5 |
Background Information
GMFB, Glia maturation factor beta, is a 17kd protein which initially identified as a nerve growth factor implicated in nervous system development. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. GMFB causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells. GMFB can be considered as a prognostic predictor for SOC(Serous ovarian carcinoma) patients. This kit is used to quantify GMFB level in vivo.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Glia maturation factor beta, glia maturation factor, beta, GMF, GMF beta, GMFB |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Surg Neurol Int Cytokines, brain proteins, and growth factors in acute stroke patients: A pilot study. |