Human GMFB ELISA Kit

Sensitivity

23.8 pg/mL

Range

125-8000 pg/mL

Reacted Species

Human

Cat no : KE00001

Synonyms

Glia maturation factor beta, glia maturation factor, beta, GMF, GMF beta, GMFB



Product Information

KE00001 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GMFB ELISA kit is to be used to detect and quantify protein levels of endogenous GMFB. The assay recognizes human GMFB. An antibody specific for GMFB has been pre-coated onto the microwells. The GMFB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for GMFB is added to detect the captured GMFB protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human GMFB ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 23.8 pg/mL
Range 125-8000 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plamsa87%75%-102%
Cell culture supernatants91%83%-99%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1206,361.0413.56.5
2202,641.6143.35.4
3201,467.3102.07.0

InterAssay

Samplenmean ( pg/mL)SDCV%
1245,734.9753.213.1
2242,553.0164.06.4
3241,509.783.05.5

Background Information

GMFB, Glia maturation factor beta, is a 17kd protein which initially identified as a nerve growth factor implicated in nervous system development. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. GMFB causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells. GMFB can be considered as a prognostic predictor for SOC(Serous ovarian carcinoma) patients. This kit is used to quantify GMFB level in vivo.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms Glia maturation factor beta, glia maturation factor, beta, GMF, GMF beta, GMFB