Recombinant Human IFNAR1 protein (Myc Tag, His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

not tested

Cat no : Eg0156

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Validation Data Gallery

• 

  Purity of Recombinant Human IFNAR1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human IFNAR1 protein Lys28-Lys436 (Accession# P17181-1) with a Myc tag and a His tag at the C-terminus.
• GeneID: 3454
• Accession: P17181-1
• PredictedSize: 52.1 kDa
• SDS-PAGE: 65-90 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interferon alpha and beta receptor subunit 1 (IFNAR1) is a type I membrane protein that associates with IFNAR2 to form the receptor for type I interferons, including interferons-alpha, -beta, and -lambda. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.

References:

1. G Uzé, et al. (2007) Curr Top Microbiol Immunol. 316:71-95.
2. Nicole A de Weerd, et al. (2012) Immunol Cell Biol. 90(5):483-91. 
3. Peter E Kima. (2014) Microbes Infect. 16(9):721-6.