Human IL-28A ELISA Kit
Cat no : KE00011
Synonyms
Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL-28, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20
Validation Data Gallery
Product Information
KE00011 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL28A ELISA kit is to be used to detect and quantify protein levels of endogenous IL28A. The assay recognizes human IL28A. An antibody specific for IL28A has been pre-coated onto the microwells. The IL28A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL28A is added to detect the captured IL28A protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-28A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 282616 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 100% | 89%-109% |
| Human plamsa | 95% | 87%-102% |
| Cell culture supernatants | 101% | 88%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 357.5 | 34.2 | 9.6 |
| 2 | 20 | 89.6 | 5.1 | 5.7 |
| 3 | 20 | 23.2 | 0.9 | 4.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 375.8 | 22.8 | 6.1 |
| 2 | 24 | 95.1 | 4.4 | 4.6 |
| 3 | 24 | 23.1 | 1.0 | 4.4 |
Background Information
IL28A, also known as IFNL2, is a cytokine distantly related to type I interferons and the IL-10 family. IL-28A, IL-28B and IL-29 are three closely related cytokines classified as type III IFNs, which share many of the biological effects of type I IFNs but may have fewer side effects due to a more selective receptor distribution. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). IL28A is believed to play a significant role in the antiviral immune defense in the intestinal epithelium.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL-28, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.