Human IL-28A ELISA Kit

Sensitivity

1.0 pg/mL

Range

7.8-500 pg/mL

Reacted Species

Human

Cat no : KE00011

Synonyms

Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL-28, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20



Product Information

KE00011 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL28A ELISA kit is to be used to detect and quantify protein levels of endogenous IL28A. The assay recognizes human IL28A. An antibody specific for IL28A has been pre-coated onto the microwells. The IL28A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL28A is added to detect the captured IL28A protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human IL-28A ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine
Assay type Sandwich
Sensitivity 1.0 pg/mL
Range 7.8-500 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Urine100%89%-109%
Human plamsa95%87%-102%
Cell culture supernatants101%88%-109%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120357.534.29.6
22089.65.15.7
32023.20.94.0

InterAssay

Samplenmean ( pg/mL)SDCV%
124375.822.86.1
22495.14.44.6
32423.11.04.4

Background Information

IL28A, also known as IFNL2, is a cytokine distantly related to type I interferons and the IL-10 family. IL-28A, IL-28B and IL-29 are three closely related cytokines classified as type III IFNs, which share many of the biological effects of type I IFNs but may have fewer side effects due to a more selective receptor distribution. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). IL28A is believed to play a significant role in the antiviral immune defense in the intestinal epithelium.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL-28, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20