Human IL-8 ELISA Kit

Sensitivity

1.0 pg/ml

Range

15.6-1000 pg/mL

Reactivity

Human

Cat no : KE00006

Synonyms

C X C motif chemokine 8, CXCL8, CXCL8,IL8, GCP1, IL 8, IL8, IL-8, interleukin 8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP 1, NAP1, Neutrophil activating factor, T cell chemotactic factor



Product Information

KE00006 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL8 ELISA kit is to be used to detect and quantify protein levels of endogenous IL8. The assay recognizes human IL8. An antibody specific for IL8 has been pre-coated onto the microwells. The IL8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL8 is added to detect the captured IL8 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human IL-8 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 1.0 pg/ml
Range 15.6-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plamsa100%80%-127%
Cell culture supernatants101%87%-114%

IntraAssay

Samplenmean ( pg/ml)SDCV%
120127.35.34.1
220411.623.75.8
320804.444.75.6

InterAssay

Samplenmean ( pg/ml)SDCV%
124123.74.83.9
224420.917.14.1
324788.935.84.5

Background Information

Interleukin 8 (IL8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL8 is also known to be a potent promoter of angiogenesis. IL8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms C X C motif chemokine 8, CXCL8, CXCL8,IL8, GCP1, IL 8, IL8, IL-8, interleukin 8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP 1, NAP1, Neutrophil activating factor, T cell chemotactic factor

Publications

SpeciesSample TypeTitle
humancell culture supernatants

Drug Des Devel Ther

Cathepsin G cleaves and activates IL-36γ and promotes the inflammation of psoriasis.

Authors - Jing Guo
humancell culture supernatants

Front Oncol

Senolytics (DQ) Mitigates Radiation Ulcers by Removing Senescent Cells.

Authors - Huilan Wang
humancell culture supernatants

mBio

Intracellular Group A Streptococcus Induces Golgi Fragmentation To Impair Host Defenses through Streptolysin O and NAD-Glycohydrolase.

Authors - Takashi Nozawa
humanserum

Exp Ther Med

Analysis of curative effect of adjuvant therapy with bronchoalveolar lavage on COPD patients complicated with pneumonia.

Authors - Huan Zhao
humanserum

BMC Pregnancy Childbirth

A cross-sectional study on the correlation between cytokines in a pelvic environment and tubal factor infertility.

Authors - Jiacong Yan
humancell culture supernatants

Cytokine

An inflammatory stimulus sensitizes TRPA1 channel to increase cytokine release in human lung fibroblasts.

Authors - Jennifer Maries Go Yap