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Recombinant Human KLK6 protein (rFc Tag) (HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2826

Validation Data Gallery

  • Purity of Recombinant Human KLK6 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human KLK6 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human KLK6 was greater than 90% as determined by SEC-HPLC.

    The purity of Human KLK6 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human KLK6 protein Glu17-Lys244 (Accession# Q92876-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 5653
Accession Q92876-1
PredictedSize 51.2 kDa
SDS-PAGE 10 kDa, 44-50 kDa and 55-60 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Kallikrein-related peptidase 6 (KLK6) belongs to a family of serine proteases that are emerging as prevalent biomarkers of inflammatory and malignant diseases. Cell culture and preclinical animal model studies suggest that KLK6 may promote inflammation and autoimmunity via cleavage of the G protein–coupled protease-activated receptor 1 (PAR1) and PAR2.

References:

1. Billi AC, et al. (2020). J Clin Invest. 130(6):3151-3157.
2. Oikonomopoulou K, et al. (2006). Biol Chem. 387(6):817–824. 
3. Radulovic M, et al. (2015). Neurobiol Dis. 83:75–89.