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Recombinant Human MMP-1 protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0527

Validation Data Gallery

  • Purity of Recombinant Human MMP-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human MMP-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human MMP-1 protein Phe20-Asn469 (Accession# P03956) with a His tag at the C-terminus.
GeneID 4312
Accession P03956
PredictedSize 55.9 kDa
SDS-PAGE 55-65 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Matrix metalloproteinase 1 (MMP1), which is also known as interstitial collagenase and fibroblast collagenase is a member of the matrix metalloproteinases (MMPs) family . Proteins in this family mainly participate in the breakdown of extracellular matrix both in normal physiological processes and disease processes. Several factors contribute to the expression of MMP1, including endogenous factors, such as polymorphisms and epigenetic regulation in the promoter region of MMP1, as well as exogenous factors, such as the tumor microenvironment. A variety of MMP1 promoter genotypic polymorphisms have been reported in various ethnic populations, and their contributions to different disease risks have been explored. In the tumor microenvironment, a variety of inflammatory factors, including interleukin-8 (IL-8), IL-1β and tumor necrosis factor-α (TNF-α), have been reported to induce MMP1 expression in cancer cells.

References:

1. Shen CJ. et al. (2017) PLoS One. 12(3):e0174487. 
2. Chen Y. et al. (2019) Int J Oncol. 55(1):142-156. 
3. Young-Min SA. et al. (2001) Ann Rheum Dis. 60(9):846-51. 
4. Murawaki Y. et al. (1999) J Gastroenterol Hepatol. 14(2):138-45.