Recombinant Human NCR3 protein (Myc Tag, His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

not tested

Cat no : Eg31663

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Validation Data Gallery

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <1.0 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human NCR3 protein Leu19-Gly135 (Accession# O14931-1) with a Myc tag and a His tag at the C-terminus.
• GeneID: 259197
• Accession: O14931-1
• PredictedSize: 18.4 kDa
• Formulation: Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

NKp30 (CD337) (NCR3) is a 30kDa type I transmembrane protein characterized by a single V-type immunoglobulin (Ig) extracellular domain. NKp30 expression is mostly restricted to NK cells , although recent studies found this NCR to be present on the surface of additional immune cells, including γδ T cells and Innate Lymphoid Cells (ILCs). NKp30 is involved in NK cell-mediated killing of various tumor cell lines, virus-infected cells, and also of autologous dendritic cells (DCs) during the so-called "editing" process.

References:

1. Pende, D et al. The Journal of experimental medicine vol. 190,10 (1999): 1505-16.
2. Hudspeth, Kelly et al. Blood vol. 119,17 (2012): 4013-6.
3. Di Vito, Clara et al. Seminars in immunology vol. 41 (2019): 101272.