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Recombinant Human NPRC/NPR3 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2988

Validation Data Gallery

  • Purity of Recombinant Human NPRC was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human NPRC was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human NPRC protein Gly27-GLu481 (Accession# P17342-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 4883
Accession P17342-1
PredictedSize 76.4 kDa
SDS-PAGE 76-85 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

NPR3 (Natriuretic Peptide Receptor 3) is a widely expressed natriuretic peptide receptor that mediates natriuretic peptide degradation and plays a role in the regulation of endochondral ossification and bone growth, as well as acting as a tumor suppressor or promoter in some types of cancers. NPR3 may function differently in different types of cancers, and the up-regulation of NPR3 upregulation of NPR3 promotes the proliferation of colorectal cancer cells, and knockdown promotes the metastasis of clear cell renal cell carcinoma. It has been suggested that NPR3 may act as a new tumor suppressor by blocking the PI3K/AKT pathway and is regulated by POU2F1 transcription.

References:

1. Li S. et al. (2021) Cell Signal. 86:110074.                                                               
2. Devotta A. et al. (2023) Elife. 12:e84036.                                                                 
3. Dabaghie D. et al. (2024) Sci Rep. 14(1):11850.