Human Naïve/Memory T Cell Panel

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  1x10^6 Human PBMCs were stained with PK30011 Human Memory/Naïve T Cell Panel. CD3+ cells are gated. Parent population: Lymphocytes. Cells were not fixed.

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  1x10^6 Human PBMCs were stained with PK30011 Human Memory/Naïve T Cell Panel. CD4 and CD8 expression on CD3+ lymphocytes are shown. Cells were not fixed.

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  1x10^6 Human PBMCs were stained with PK30011 Human Memory/Naïve T Cell Panel. CD45RA and CD45RO expression on CD3+CD8+ (left) and CD3+/CD4+ (right) lymphocytes are shown. Cells were not fixed.

Cat No. PK30011

Host

Mouse

Reactivity

Human

Applications

FC

Conjugate

Various

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Product Information

Naïve T cells have not previously encountered antigen while memory T cells have and can therefore more quickly respond to subsequent encounters of that antigen.

This panel is designed for the separation of T lymphocytes (CD3 positive) into Helper T Lymphocyte (CD4 positive), Cytotoxic T Lymphocyte (CD8 positive), Naïve T Lymphocytes (CD45RA expressing) and Memory T Lymphocytes (CD45RO expressing). The PE channel is left open. 

Panel Components

Fluorochrome (ex/em)	Catalog No	Target	Clone	Isotype	Description
CoraLite Plus 405	CL405-65151	CD3	UCHT1	Mouse IgG1k	T cell marker
(399 nm/422 nm)
CoraLite Plus 647	CL647-65134	CD4	OKT4	Mouse IgG2bk	T helper cell marker
(654 nm/674 nm)
CoraLite Plus 750	CL750-65135	CD8a	OKT8	Mouse IgG2ak	Cytotoxic T cell marker
(755 nm/780 nm)
Cardinal Red	CR-65226	CD45RA	F8-11-13	Mouse IgG1k	Naïve T cell Marker
(592 nm/611 nm)
FITC Plus	FITC-65150	CD45RO	UCHL1	Mouse IgG2ak	Memory T cell Marker
(495 nm/524 nm)

Storage

Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.

Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 

Usage

Human Naïve/Memory T Cell Panel Datasheet and User Guide

Flow-Cytometry-Cell-Surface-Staining-Protocol

Cited in Article as

PK30011, Human Naïve/Memory T Cell Panel, Proteintech, IL, USA