Human TGF-beta1 ELISA Kit

Sensitivity

3.3 pg/mL

Range

15.6-1000 pg/mL

Reacted Species

Human

Cat no : KE00002

Synonyms

CED, DPD1, LAP, TGF ?1, TGF beta 1, TGF- beta 1, TGFB, TGFB1, TGFbeta



Product Information

KE00002 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TGF-beta1 ELISA kit is to be used to detect and quantify protein levels of endogenous TGF-beta1. The assay recognizes human TGF-beta1. An antibody specific for TGF-beta1 has been pre-coated onto the microwells. The TGF-beta1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for TGF-beta1 is added to detect the captured TGF-beta1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human TGF-beta1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine
Assay type Sandwich
Sensitivity 3.3 pg/mL
Range 15.6-1000 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Urine93%86%-101%
Human plasma100%81%-111%
Cell culture supernatants105%93%-115%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120632.628.24.5
22081.23.94.8
32018.50.94.9

InterAssay

Samplenmean ( pg/mL)SDCV%
124659.662.09.4
22483.34.55.5
32415.50.32.0

Background Information

TGF-beta is a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. TGF-beta is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells. TGF-beta acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. TGF-beta plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. TGF-beta appears to promote late stage progression and metastasis in some cancers.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms CED, DPD1, LAP, TGF ?1, TGF beta 1, TGF- beta 1, TGFB, TGFB1, TGFbeta

Publications

SpeciesSample TypeTitle
humanserum

Gynecol Oncol

Platelets promote invasion and induce epithelial to mesenchymal transition in ovarian cancer cells by TGF-β signaling pathway.

Authors - Yi Guo
plasma

J Nutr Biochem

Can the dietary fat type facilitate memory impairments in adulthood? A comparative study between Mediterranean and Western-based diet in rats.

Authors - Priscila Haygert
humancell culture supernatants

Regen Ther

A cancer invasion model of cancer-associated fibroblasts aggregates combined with TGF-β1 release system.

Authors - Teruki Nii
humanserum

Oncol Lett

Effect of combined propofol-sevoflurane anesthesia on immune function in pediatric patients with acute lymphoblastic leukemia.

Authors - Niu Di

Oncol Lett

Effect of combined propofol-sevoflurane anesthesia on immune function in pediatric patients with acute lymphoblastic leukemia.

Authors - Niu Di
humanserum

Climacteric

Evaluation of CD4+CD25+FOXP3+ regulatory T cells and FOXP3 mRNA in premature ovarian insufficiency.

Authors - J Xiong