Human TNF-alpha ELISA Kit

Sensitivity

1.0 pg/mL

Range

31.25-2000 pg/mL

Reacted Species

Human

Cat no : KE00068

Synonyms

Cachectin, DIF, TNF, TNFA, TNF-a, TNFSF2



Product Information

KE00068 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNF-α ELISA kit is to be used to detect and quantify protein levels of endogenous TNF-α. The assay recognizes human TNF-α. An antibody specific for TNF-α has been pre-coated onto the microwells. The TNF-α protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for TNF-α is added to detect the captured TNF-α protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human TNF-alpha ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 1.0 pg/mL
Range 31.25-2000 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plasma77%70%-86%
Cell culture supernatants100%81%-126%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120365.811.03.0
220747.842.25.6
3201,508.196.96.4

InterAssay

Samplenmean ( pg/mL)SDCV%
124392.221.05.4
224774.433.54.3
3241,572.561.93.9

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression and inflammatory bowel disease (IBD).

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms Cachectin, DIF, TNF, TNFA, TNF-a, TNFSF2

Publications

SpeciesTitle
human

EBioMedicine

H. pylori infection induced BMAL1 expression and rhythm disorder aggravate gastric inflammation.

Authors - Tongyu Li
human

Exp Ther Med

Changes in expression of p53 and inflammatory factors in patients with ulcerative colitis.

Authors - Hui Su
human

Am J Transl Res

Novel apoE receptor mimetics reduce LPS-induced microglial inflammation.

Authors - Likun Wang
human

Med Sci Monit

Protective Effects of Dexmedetomidine and Oxycodone in Patients Undergoing Limb Ischemia-Reperfusion.

Authors - Wenjie Cheng
human

Exp Ther Med

Analysis of curative effect of adjuvant therapy with bronchoalveolar lavage on COPD patients complicated with pneumonia.

Authors - Huan Zhao
human

Oncol Lett

Effect of dexmedetomidine anesthesia on perioperative levels of TNF-α and IL-6 in patients with ovarian cancer.

Authors - Maodong Liu