Human Tie2 ELISA Kit
Cat no : KE00242
Synonyms
Angiopoietin 1 receptor, CD202B, hTIE2, p140 TEK, TEK, TIE 2, TIE2, VMCM, VMCM1
Validation Data Gallery
Product Information
KE00242 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Tie2 ELISA kit is to be used to detect and quantify protein levels of endogenous Tie2. The assay recognizes human Tie2. An antibody specific for Tie2 has been pre-coated onto the microwells. The Tie2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Tie2 is added to detect the captured Tie2 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Tie2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum and Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7010 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 106% | 83%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 34.3 | 0.9 | 2.7 |
| 2 | 20 | 133.2 | 5.7 | 4.3 |
| 3 | 20 | 516.2 | 11.3 | 2.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 30.9 | 2.2 | 7.1 |
| 2 | 24 | 128.9 | 3.9 | 3.0 |
| 3 | 24 | 495.6 | 20.2 | 4.1 |
Background Information
Tie2 (also known as TEK) is a tyrosine-protein kinase expressed almost exclusively on endothelial cells. It contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. Tie2 acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Mutations in the gene of Tie2 are associated with inherited venous malformations of the skin and mucous membranes. Tie2 can also exist as a soluble form (sTie2) which is released from endothelial cells and present in human blood. The concentration of sTie2 is increased in a range of diseases, including peripheral arterial disease and myocardial infarction.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Angiopoietin 1 receptor, CD202B, hTIE2, p140 TEK, TEK, TIE 2, TIE2, VMCM, VMCM1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.