Recombinant Human XCL1 protein (rFc Tag)

Species

Human

Purity

>80 %, SDS-PAGE

Tag

C-rFc

Activity

not tested

Cat no : Eg2933

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Validation Data Gallery

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Product Information

• Purity: >80 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human XCL1 protein Val22-Gly114 (Accession# P47992) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 6375
• Accession: P47992
• PredictedSize: 36.3 KDa
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The human chemokine XCL1 is a metamorphic protein that exists as a mixture of two equally populated native state conformations under near-physiologic conditions. XCL1 interconverts spontaneously and reversibly between its two structures, which are (1) the canonical α − β chemokine fold, and (2) a 4-stranded β-sheet that forms a dimer.

References:

1. Tuinstra RL, et al. Proc. Natl. Acad. Sci. U. S. A 2008, 105, 5057.
2. Kuloglu ES, et al. Biochemistry 2001, 40, 12486.
3. Kuloglu ES, et al. J. Biol. Chem 2002, 277, 17863.