IBA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

IBA1 Polyclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

295

Reviews (1)

Cat no : 10904-1-AP

Synonyms

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

---

Validation Data Gallery

• 

  WB analysis of THP-1 using 10904-1-AP

  WB result of IBA1 antibody (10904-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and IBA1 knockout THP-1 cells.

• 

  IHC staining of rat brain using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 10904-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using IBA1 antibody (10904-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of THP-1 using 10904-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human IBA1 (10904-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IHC detected in: rat brain tissue, human tonsillitis tissue, human liver tissue, mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue
• Positive FC detected in: THP-1 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 5 publications below
• WB: See 96 publications below
• IHC: See 74 publications below
• IF: See 153 publications below
• IP: See 1 publications below

Product Information

10904-1-AP targets IBA1 in IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, hamster, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IBA1 fusion protein Ag1363
• Full Name: allograft inflammatory factor 1
• Calculated molecular weight: 17 kDa
• GenBank accession number: BC009474
• Gene symbol: AIF1
• Gene ID (NCBI): 199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the molecular weight of IBA1?

The molecular weight of IBA1 is 16.7 kD.

 What is IBA1?

Ionized calcium-binding adaptor molecule 1 (IBA1), also known as Allograft inflammatory factor-1 (AIF-1), is an inflammation-responsive scaffold protein expressed and secreted from macrophages. Microglia response factor (MRF-1) and daintain are also similar, and likely identical, proteins (PMIDs: 29749461, 9630473, 23792284).

 What is the function of IBA1?

IBA1 is necessary for macrophage survival, and it is also a key molecule in proinflammatory activity (PMID: 29749461).

 Where is IBA1 localized?

IBA1 is a cytoplasmic protein, often expressed in immunocytes, macrophages, and microglia. It can be used as a marker for normal (not 'dark') microglia in brain tissue, as IBA1 is expressed by all microglial cell subpopulations (PMIDs: 29749461, 11943136, 26847266, 9630473).

 Is IBA1 upregulated in active immunophages?

Yes; its expression is associated with inflammatory activity (PMID: 29749461).

 How do IBA1-positive microglia differ from microglia that express less or no IBA1?

'Dark' microglia is a recently described phenotype associated with Alzheimer's disease pathology and chronic stress; these dark microglia express decreased IBA1 (often punctiform), and show distinct ultrastructural differences from 'normal' microglia as well as condensed and electron-dense cytoplasm and nucleoplasm. Normal microglia generally display strong, diffuse expression of IBA1. IBA1-positive microglial processes in normal conditions also have a strong tendency to exclusively contact synaptic elements, such as axon terminals, dendritic spines, and synaptic clefts (PMIDs: 29992181, 26847266).

 How is IBA1 related to Alzheimer's disease and pain?

Exacerbated immunoactivity of IBA1, particularly in proximity to amyloid plaques, is prominently featured in AD pathology. IBA1, along with other microglial markers, are also associated with pain, and robust microglial reactions often follow spinal cord injury (PMIDs: 29992181, 23792284).

Publications

Species	Application	Title
	IHC	Acta Neuropathol; Splicing repression is a major function of TDP-43 in motor neurons.; Authors - Aneesh Donde
		ACS Nano; Nanozyme-Based Bandage with Single-Atom Catalysis for Brain Trauma.; Authors - Ruijuan Yan
human	IHC	Brain; MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.; Authors - Bing-Lin Zhu
mouse	WB	Brain; Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.; Authors - Yun Xia
human,mouse	IF	Redox Biol; MG53 protein rejuvenates hUC-MSCs and facilitates their therapeutic effects in AD mice by activating Nrf2 signaling pathway.; Authors - Shanshan Ma
mouse	WB	Mol Neurodegener; Islet amyloid polypeptide cross-seeds tau and drives the neurofibrillary pathology in Alzheimer's disease.; Authors - Guoxin Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

NX (Verified Customer) (02-27-2023)

A clean antibody to detect endogenous Iba1 from mouse brain lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse brain lysate

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C

TNF Alpha Monoclonal antibody

60291-1-Ig

TNF Alpha Polyclonal antibody

17590-1-AP

GFAP Polyclonal antibody

16825-1-AP

CD11B/Integrin Alpha M Polyclonal antibody

20991-1-AP

CD45 Polyclonal antibody

20103-1-AP

CD68-Specific Polyclonal antibody

16192-1-AP