ID1 Polyclonal antibody, PBS Only

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  WB analysis of PC-3 using 18475-1-AP (same clone as 18475-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18475-1-AP (ID1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18475-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 18475-1-AP (same clone as 18475-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18475-1-AP (ID1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18475-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 18475-1-AP (same clone as 18475-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18475-1-AP (ID1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18475-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 18475-1-AP (same clone as 18475-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18475-1-AP (ID1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18475-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 18475-1-AP (same clone as 18475-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ID1 antibody (18475-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 18475-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ID1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 18475-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Inhibitor of DNA binding 1, Inhibitor of differentiation 1, ID, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24

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Product Information

18475-1-PBS targets ID1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ID1 fusion protein Ag13359
• Full Name: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC000613
• Gene Symbol: ID1
• Gene ID (NCBI): 3397
• RRID: AB_2248812
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41134
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ID (inhibitor of DNA binding) proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID1 proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID proteins contribute to cell growth, senescence, differentiation, and angiogenesis. Inhibitor of differentiation or DNA binding-1 (ID1) interacts with the transactivation domain of p65 (p65TAD) both in vitro and in vivo. The molecular weight of ID1 is about 20 kDa.