Tested Applications
| Positive WB detected in | human placenta tissue, IFN gamma treated HeLa cells |
| Positive IF/ICC detected in | IFN gamma treated HepG2 cells, SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13268-1-AP targets IDO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3953 Product name: Recombinant human IDO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-403 aa of BC027882 Sequence: MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG Predict reactive species |
| Full Name | indoleamine 2,3-dioxygenase 1 |
| Calculated Molecular Weight | 403 aa, 45 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC027882 |
| Gene Symbol | IDO1 |
| Gene ID (NCBI) | 3620 |
| RRID | AB_2123444 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14902 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IDO1 antibody 13268-1-AP | Download protocol |
| WB protocol for IDO1 antibody 13268-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-IDO1 (Cat# 13268-1-AP) has accumulated 123 citations published in journals including Nature Communications, Cancer Research, Hepatology, Advanced Science, Redox Biology, Theranostics, Cell Discovery, and Journal of Immunotherapy of Cancer. Key research fields span cancer immunology and immunosuppression, tryptophan–kynurenine metabolism, neuroinflammation and depression, liver disease, gut microbiota modulation, ferroptosis, autoimmune disorders, and pulmonary fibrosis.
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Tryptophan 2,3-dioxygenase-positive matrix fibroblasts fuel breast cancer lung metastasis via kynurenine-mediated ferroptosis resistance of metastatic cells and T cell dysfunction | ||
Cancer Res CD58 Alterations Govern Antitumor Immune Responses by Inducing PDL1 and IDO in Diffuse Large B-Cell Lymphoma | ||
Nat Commun A circuit linking dentate gyrus and retrosplenial granular cortex regulates fracture healing in male mice. | ||
Nat Commun Induction of the hepatic aryl hydrocarbon receptor by alcohol dysregulates autophagy and phospholipid metabolism via PPP2R2D | ||
Hepatology Indoleamine-2, 3-dioxygenase as an effector and an indicator of protective immune responses in patients with acute hepatitis B.
| ||
Cell Discov Single-cell transcriptomics reveals intestinal cell heterogeneity and identifies Ep300 as a potential therapeutic target in mice with acute liver failure |
Reviews
What customers say
Both reviewers used this antibody for Western Blot and reported positive results. One user (5/5) found it worked well with a 1-hour room temperature incubation at a 1:2000 dilution on A549 cells. The other (4/5) expressed strong satisfaction with both the antibody performance and company support, using it at 1:1000 on macrophages and HeLa cells. Overall, users report reliable WB performance across different cell types at standard dilutions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Christopher (Verified Customer) (01-28-2022) | Worked well with a 1 hour incubation at room temperature.
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Hala (Verified Customer) (09-18-2020) | I am very satisfied of this antibody and of the interaction with your company
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