|Positive WB detected in||human placenta tissue, IFN gamma treated HeLa cells|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13268-1-AP targets IDO1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IDO1 fusion protein Ag3953|
|Full Name||indoleamine 2,3-dioxygenase 1|
|Calculated molecular weight||403 aa, 45 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||BC027882|
|Gene ID (NCBI)||3620|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).
Indoleamine-2, 3-dioxygenase as an effector and an indicator of protective immune responses in patients with acute hepatitis B.
ACS Appl Mater Interfaces
Mitigation Effects of Selenium Nanoparticles on Depression-Like Behavior Induced by Fluoride in Mice via the JAK2-STAT3 Pathway.
J Immunother Cancer
GCH1 induces immunosuppression through metabolic reprogramming and IDO1 upregulation in triple-negative breast cancer.
J Immunother Cancer
Tryptophan potentiates CD8+ T cells against cancer cells by TRIP12 tryptophanylation and surface PD-1 downregulation.
Enhanced cancer therapy through synergetic photodynamic/immune checkpoint blockade mediated by a liposomal conjugate comprised of porphyrin and IDO inhibitor.
Exosome-like nanoparticles from Mulberry bark prevent DSS-induced colitis via the AhR/COPS8 pathway.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Christopher (Verified Customer) (01-28-2022)
Worked well with a 1 hour incubation at room temperature.
Hala (Verified Customer) (09-18-2020)
I am very satisfied of this antibody and of the interaction with your company