IDO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

IDO1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

56

Reviews (2)

Cat no : 13268-1-AP

Synonyms

CD107B, IDO, IDO 1, IDO1, INDO, indoleamine 2,3 dioxygenase 1, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase

---

Validation Data Gallery

• 

  WB analysis using 13268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13268-1-AP (IDO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13268-1-AP

  Human placenta were subjected to SDS PAGE followed by western blot with 13268-1-AP (IDO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 13268-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13268-1-AP (IDO1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13268-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13268-1-AP (IDO1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: human placenta tissue, IFN gamma treated HeLa cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 44 publications below
• IHC: See 10 publications below
• IF: See 7 publications below

Product Information

13268-1-AP targets IDO1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IDO1 fusion protein Ag3953
• Full Name: indoleamine 2,3-dioxygenase 1
• Calculated Molecular Weight: 403 aa, 45 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC027882
• Gene Symbol: IDO1
• Gene ID (NCBI): 3620
• RRID: AB_2123444
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).

Publications

Species	Application	Title
human	WB	Hepatology; Indoleamine-2, 3-dioxygenase as an effector and an indicator of protective immune responses in patients with acute hepatitis B.; Authors - Sachiyo Yoshio ; KO Validated
mouse	WB	Acta Biomater; Blockage of the IDO1 pathway by charge-switchable nanoparticles amplifies immunogenic cell death for enhanced cancer immunotherapy.; Authors - Menghao Shi
mouse	WB	ACS Appl Mater Interfaces; Mitigation Effects of Selenium Nanoparticles on Depression-Like Behavior Induced by Fluoride in Mice via the JAK2-STAT3 Pathway.; Authors - Jiarong Yang
human	WB	J Immunother Cancer; GCH1 induces immunosuppression through metabolic reprogramming and IDO1 upregulation in triple-negative breast cancer.; Authors - Jin-Li Wei
human	WB	J Immunother Cancer; Tryptophan potentiates CD8+ T cells against cancer cells by TRIP12 tryptophanylation and surface PD-1 downregulation.; Authors - Rui Qin
human	WB	Theranostics; Enhanced cancer therapy through synergetic photodynamic/immune checkpoint blockade mediated by a liposomal conjugate comprised of porphyrin and IDO inhibitor.; Authors - Zeqian Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Christopher (Verified Customer) (01-28-2022)

Worked well with a 1 hour incubation at room temperature.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: A549

IDO1 Antibody Western Blot validation (1:2000 dilution) in A549 (Cat no:13268-1-AP)

FH

Hala (Verified Customer) (09-18-2020)

I am very satisfied of this antibody and of the interaction with your company

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: macrophages and hela cells

IDO1 Antibody Western Blot validation (1:1000 dilution) in macrophages and hela cells (Cat no:13268-1-AP)

Related Products

IFN Gamma Polyclonal antibody

15365-1-AP

TDO2 Polyclonal antibody

15880-1-AP

FOXP3 Polyclonal antibody

22228-1-AP

KMO Polyclonal antibody

10698-1-AP

HO-1/HMOX1 Polyclonal antibody

10701-1-AP

IL-4 Monoclonal antibody

66142-1-Ig