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  • KD/KO Validated

IDO1 Polyclonal antibody

IDO1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (3)





Cat no : 13268-1-AP


CD107B, IDO, IDO 1, IDO1, INDO, indoleamine 2,3 dioxygenase 1, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase

Tested Applications

Positive WB detected inhuman placenta tissue, IFN gamma treated HeLa cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13268-1-AP targets IDO1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IDO1 fusion protein Ag3953
Full Name indoleamine 2,3-dioxygenase 1
Calculated Molecular Weight 403 aa, 45 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC027882
Gene Symbol IDO1
Gene ID (NCBI) 3620
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).


Product Specific Protocols
WB protocol for IDO1 antibody 13268-1-APDownload protocol
IHC protocol for IDO1 antibody 13268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Indoleamine-2, 3-dioxygenase as an effector and an indicator of protective immune responses in patients with acute hepatitis B.

Authors - Sachiyo Yoshio
  • KO Validated

Acta Biomater

Blockage of the IDO1 pathway by charge-switchable nanoparticles amplifies immunogenic cell death for enhanced cancer immunotherapy.

Authors - Menghao Shi

ACS Appl Mater Interfaces

Mitigation Effects of Selenium Nanoparticles on Depression-Like Behavior Induced by Fluoride in Mice via the JAK2-STAT3 Pathway.

Authors - Jiarong Yang

J Immunother Cancer

GCH1 induces immunosuppression through metabolic reprogramming and IDO1 upregulation in triple-negative breast cancer.

Authors - Jin-Li Wei

J Immunother Cancer

Tryptophan potentiates CD8+ T cells against cancer cells by TRIP12 tryptophanylation and surface PD-1 downregulation.

Authors - Rui Qin


Enhanced cancer therapy through synergetic photodynamic/immune checkpoint blockade mediated by a liposomal conjugate comprised of porphyrin and IDO inhibitor.

Authors - Zeqian Huang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Christopher (Verified Customer) (01-28-2022)

Worked well with a 1 hour incubation at room temperature.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: A549
IDO1 Antibody Western Blot validation (1:2000 dilution) in A549 (Cat no:13268-1-AP)

Hala (Verified Customer) (09-18-2020)

I am very satisfied of this antibody and of the interaction with your company

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: macrophages and hela cells
IDO1 Antibody Western Blot validation (1:1000 dilution) in macrophages and hela cells (Cat no:13268-1-AP)