IFNAR1 Recombinant monoclonal antibody, PBS Only

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  WB analysis of HeLa using 83002-4-RR (same clone as 83002-4-PBS)

  WB result of IFNAR1 antibody (83002-4-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFNAR1 transfected HeLa cells. This data was developed using the same antibody clone with 83002-4-PBS in a different storage buffer formulation.

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  WB analysis using 83002-4-RR (same clone as 83002-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83002-4-RR (IFNAR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83002-4-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 83002-4-RR (same clone as 83002-4-PBS)

  1x10^6 Jurkat cells were surface stained with 0.25 ug IFNAR1 Recombinant antibody (83002-4-RR, Clone:230549C8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 83002-4-PBS in a different storage buffer formulation.

• 

  FC experiment of K562 using 83002-4-RR (same clone as 83002-4-PBS)

  1x10^6 K562 were surface stained with 0.25 ug IFNAR1 Recombinant antibody (83002-4-RR, Clone:230549C8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 83002-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83002-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83002-4-RR against Human IFNAR1 were performed. The affinity constant is 2.04 nM.

• Featured Product
• KD/KO Validated

IFNAR1 Uni-rAb^® Recombinant Antibody for WB, FC, Indirect ELISA

Cat No. 83002-4-PBS

Clone No.230549C8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, Indirect ELISA

Synonyms

230549C8, AVP, CRF2 1, CRF2-1, Cytokine receptor class-II member 1

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Product Information

83002-4-PBS targets IFNAR1 in WB, FC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0156

  Product name: Recombinant Human IFNAR1 protein (Myc Tag, His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: Myc & 6*His

  Domain: 28-436 aa of BC021825

  Sequence: KNLKSPQKVEVDIIDDNFILRWNRSDESVGNVTFSFDYQKTGMDNWIKLSGCQNITSTKCNFSSLKLNVYEEIKLRIRAEKENTSSWYEVDSFTPFRKAQIGPPEVHLEAEDKAIVIHISPGTKDSVMWALDGLSFTYSLVIWKNSSGVEERIENIYSRHKIYKLSPETTYCLKVKAALLTSWKIGVYSPVHCIKTTVENELPPPENIEVSVQNQNYVLKWDYTYANMTFQVQWLHAFLKRNPGNHLYKWKQIPDCENVKTTQCVFPQNVFQKGIYLLRVQASDGNNTSFWSEEIKFDTEIQAFLLPPVFNIRSLSDSFHIYIGAPKQSGNTPVIQDYPLIYEIIFWENTSNAERKIIEKKTDVTVPNLKPLTVYCVKARAHTMDEKLNKSSVFSDAVCEKTKPGNTSK

• Full Name: interferon (alpha, beta and omega) receptor 1
• Calculated Molecular Weight: 557 aa, 64 kDa
• Observed Molecular Weight: 80-140 kDa
• GenBank Accession Number: BC021825
• Gene Symbol: IFNAR1
• Gene ID (NCBI): 3454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P17181
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Interferon alpha and beta receptor subunit 1 (IFNAR1) is a type I membrane protein that associates with IFNAR2 to form the receptor for type I interferons, including interferons-alpha, -beta, and -lambda. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.