IGF1R/CD221 Recombinant monoclonal antibody

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  WB analysis using 84385-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84385-3-RR (IGF1R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 84385-3-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IGF1R antibody (84385-3-RR, Clone: 241678B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84385-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84385-3-RR against Human IGF1R were performed. The affinity constant is 0.239 nM.

IGF1R/CD221 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84385-3-RR

Clone No.241678B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

IGF 1 receptor, IGF1R, IGF-I receptor, IGFIR, IGFR

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Tested Applications

• Positive WB detected in: MCF-7 cells, A549 cells, L02 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84385-3-RR targets IGF1R/CD221 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: IGF I receptor
• Calculated Molecular Weight: 1367 aa, 155 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC113612
• Gene Symbol: IGF1R
• Gene ID (NCBI): 3480
• RRID: AB_3671921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P08069
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is translated as ~220 kDa pro-receptors (PMID: 33969359). IGF1R is a heterotetrameric receptor, composed of two alpha subunits (~130 kDa) and two beta subunits (~100-120 kDa) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.