IGF1R Beta Chain Monoclonal antibody

IGF1R Beta Chain Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

3C6A11

Cat no : 66283-1-Ig

Synonyms

CD221, IGF I receptor, IGF1R, IGF1R-Specific, IGFIR, IGF-IR beta, IGFR, JTK13



Tested Applications

Positive WB detected inMCF-7 cells, HepG2 cells, HeLa cells, HUVEC cells, A549 cells, HEK-293 cells, ROS1728 cells, C6 cells
Positive IHC detected inhuman breast cancer tissue, human endometrial cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

66283-1-Ig targets IGF1R Beta Chain in WB, IHC,ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name IGF I receptor
Calculated molecular weight 155 kDa
Observed molecular weight 130-140 kDa
GenBank accession numberNM_000875
Gene symbol IGF1R
Gene ID (NCBI) 3480
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGF1R, also named as CD221, belongs to the protein kinase superfamily, Tyr protein kinase family and INS receptor subfamily. IGF1R binds INS-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, IGF1R binds IGF1. The hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by INS, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and INS. In contrast, the hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for INS. Defects in IGF1R may be a cause in some cases of resistance to INS-like growth factor 1 (IGF1 resistance). This antibody raised against a synthesized polypeptide (CGVLVLRASFDERQPYAH) corresponding to 1332-1348aa of human IGF1R protein recognizes beta chain of IGF1R.

Protocols

Product Specific Protocols
WB protocol for IGF1R Beta Chain antibody 66283-1-IgDownload protocol
IHC protocol for IGF1R Beta Chain antibody 66283-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur J Obstet Gynecol Reprod Biol

Comprehensive assessment the expression of core elements related to IGFIR/PI3K pathway in granulosa cells of women with polycystic ovary syndrome.

Authors - Tingting He
humanWB

Drug Deliv

Codelivery of DOX and siRNA by folate-biotin-quaternized starch nanoparticles for promoting synergistic suppression of human lung cancer cells.

Authors - Liangping Li
ratWB

Behav Brain Res

Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.

Authors - Shuai Lian
humanWB

Cell Signal

Cell surface GRP78 facilitates hepatoma cells proliferation and migration by activating IGF-IR.

Authors - Yancun Yin