|Positive WB detected in||MCF-7 cells, HepG2 cells, HeLa cells, HUVEC cells, A549 cells, HEK-293 cells, ROS1728 cells, C6 cells|
|Positive IHC detected in||human breast cancer tissue, human endometrial cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66283-1-Ig targets IGF1R Beta Chain in WB, IHC,ELISA applications and shows reactivity with human, rat samples.
|Tested Reactivity||human, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Mouse / IgG1|
|Full Name||IGF I receptor|
|Calculated molecular weight||155 kDa|
|Observed molecular weight||130-140 kDa|
|GenBank accession number||NM_000875|
|Gene ID (NCBI)||3480|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IGF1R, also named as CD221, belongs to the protein kinase superfamily, Tyr protein kinase family and INS receptor subfamily. IGF1R binds INS-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, IGF1R binds IGF1. The hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by INS, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and INS. In contrast, the hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for INS. Defects in IGF1R may be a cause in some cases of resistance to INS-like growth factor 1 (IGF1 resistance). This antibody raised against a synthesized polypeptide (CGVLVLRASFDERQPYAH) corresponding to 1332-1348aa of human IGF1R protein recognizes beta chain of IGF1R.
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