IGF1R Beta Chain Monoclonal antibody
IGF1R Beta Chain Monoclonal Antibody for IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3C6A11
Cat no : 66283-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, HepG2 cells, HeLa cells, HUVEC cells, A549 cells, HEK-293 cells, ROS1728 cells, C6 cells |
| Positive IHC detected in | human breast cancer tissue, human endometrial cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
Product Information
66283-1-Ig targets IGF1R Beta Chain in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | IGF I receptor |
| Calculated molecular weight | 155 kDa |
| Observed molecular weight | 130-140 kDa |
| GenBank accession number | NM_000875 |
| Gene symbol | IGF1R |
| Gene ID (NCBI) | 3480 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IGF1R, also named as CD221, belongs to the protein kinase superfamily, Tyr protein kinase family and INS receptor subfamily. IGF1R binds INS-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, IGF1R binds IGF1. The hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by INS, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and INS. In contrast, the hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for INS. Defects in IGF1R may be a cause in some cases of resistance to INS-like growth factor 1 (IGF1 resistance). This antibody raised against a synthesized polypeptide (CGVLVLRASFDERQPYAH) corresponding to 1332-1348aa of human IGF1R protein recognizes beta chain of IGF1R.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IGF1R Beta Chain antibody 66283-1-Ig | Download protocol |
| IHC protocol for IGF1R Beta Chain antibody 66283-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Thyroid A MULTICENTER, SINGLE-BLIND, CASE-CONTROL, IMMUNOHISTOCHEMICAL STUDY OF ORBITAL TISSUE IN THYROID EYE DISEASE | ||
Cell Signal Cell surface GRP78 facilitates hepatoma cells proliferation and migration by activating IGF-IR. | ||
Behav Brain Res Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats. | ||
Biosci Biotechnol Biochem Black rice bran intake reduces phosphorylated tau levels and enhances insulin signaling in the brain of aged normal mice | ||
Eur J Obstet Gynecol Reprod Biol Comprehensive assessment the expression of core elements related to IGFIR/PI3K pathway in granulosa cells of women with polycystic ovary syndrome. | ||
Drug Deliv Codelivery of DOX and siRNA by folate-biotin-quaternized starch nanoparticles for promoting synergistic suppression of human lung cancer cells. |
