IGF1R/CD221 Monoclonal antibody, PBS Only

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  WB analysis using 66283-1-Ig (same clone as 66283-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66283-1-Ig (same clone as 66283-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66283-1-Ig (same clone as 66283-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66283-1-Ig (same clone as 66283-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  WB analysis of HUVEC using 66283-1-Ig (same clone as 66283-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66283-1-Ig (same clone as 66283-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66283-1-Ig (same clone as 66283-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 66283-1-Ig (same clone as 66283-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 66283-1-Ig (same clone as 66283-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66283-1-Ig (same clone as 66283-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66283-1-Ig (same clone as 66283-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

IGF1R/CD221 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66283-1-PBS

Clone No.3C6A11

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

IGF 1 receptor, IGF1R, IGF-I receptor, IGFIR, IGFR

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Product Information

66283-1-PBS targets IGF1R/CD221 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: IGF I receptor
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: NM_000875
• Gene Symbol: IGF1R
• Gene ID (NCBI): 3480
• RRID: AB_2881666
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P08069
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is translated as ~220 kDa pro-receptors (PMID: 33969359). IGF1R is a heterotetrameric receptor, composed of two alpha subunits (~130 kDa) and two beta subunits (~100-120 kDa) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.