Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, mouse placenta tissue, BxPC-3 cells, NIH/3T3 cells
|Positive IP detected in
|Positive IHC detected in
|human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:2000-1:10000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
14642-1-AP targets IGF2BP3 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|IGF2BP3 fusion protein Ag6226
|insulin-like growth factor 2 mRNA binding protein 3
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.
|Product Specific Protocols
|WB protocol for IGF2BP3 antibody 14642-1-AP
|IHC protocol for IGF2BP3 antibody 14642-1-AP
|IF protocol for IGF2BP3 antibody 14642-1-AP
|IP protocol for IGF2BP3 antibody 14642-1-AP
|Click here to view our Standard Protocols
SIRT6 Suppresses Pancreatic Cancer through Control of Lin28b.
Lin28b is sufficient to drive liver cancer and necessary for its maintenance in murine models.
Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.
Nat Cell Biol
N6-methyladenosine regulates maternal RNA maintenance in oocytes and timely RNA decay during mouse maternal-to-zygotic transition.
m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3
Demethylase ALKBH5 suppresses invasion of gastric cancer via PKMYT1 m6A modification.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
honglei (Verified Customer) (01-17-2023)
This antibody can be used for immunofluorescence and mainly localizes in the nucleus
Jessica (Verified Customer) (10-29-2018)
Also binds exogenous expressed IGF2BP1-GFP protein. I also get a double band around 70 kDa that is likely IGF2BP1 and IGF2BP3. This antibody is not specific.