IGF2BP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

IGF2BP3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

37

Reviews (1)

Cat no : 14642-1-AP

Synonyms

hKOC, IGF II mRNA binding protein 3, IGF2 mRNA binding protein 3, IGF2BP3, IMP 3, IMP3, KOC1, VICKZ family member 3, VICKZ3

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Validation Data Gallery

• 

  WB analysis using 14642-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14642-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14642-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 14642-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 14642-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14642-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14642-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14642-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment using 14642-1-AP

  IP result of anti-IGF2BP3 (IP:14642-1-AP, 5ug; Detection:14642-1-AP 1:2000) with HEK-293 cells lysate 1040 ug.

• 

  IHC staining of human pancreas using 14642-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14642-1-AP (IGF2BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 14642-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14642-1-AP (IGF2BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 14642-1-AP

  Immunofluorescent analysis of MCF-7 cells, using IGF2BP3 antibody 14642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse placenta tissue, HeLa cells, HepG2 cells, BxPC-3 cells, mouse brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 14 publications below
• WB: See 29 publications below
• IHC: See 8 publications below
• IF: See 2 publications below
• RIP: See 7 publications below

Product Information

14642-1-AP targets IGF2BP3 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGF2BP3 fusion protein Ag6226
• Full Name: insulin-like growth factor 2 mRNA binding protein 3
• Calculated molecular weight: 64 kDa
• Observed molecular weight: 64 kDa
• GenBank accession number: BC065269
• Gene symbol: IGF2BP3
• Gene ID (NCBI): 10643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IGF2BP3, also named as IMP3, KOC1 and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.

Publications

Species	Application	Title
human	WB	J Cell Physiol; FNDC3B circular RNA promotes the migration and invasion of gastric cancer cells via the regulation of E-cadherin and CD44 expression.; Authors - Yuling Hong
human	WB	J Cell Mol Med; Wilms' tumour 1-associating protein inhibits endothelial cell angiogenesis by m6A-dependent epigenetic silencing of desmoplakin in brain arteriovenous malformation.; Authors - Lin-Jian Wang ; KD Validated
human	WB	Int J Mol Sci; EIF4G1 and RAN as Possible Drivers for Malignant Pleural Mesothelioma.; Authors - Irene Dell'Anno ; KD Validated
human	IHC	Oncoscience; Expression of the cancer testis antigen IGF2BP3 in colorectal cancers; IGF2BP3 holds promise as a specific immunotherapy target.; Authors - Hmc Shantha Kumara
human	WB,IHC	J Exp Clin Cancer Res; RNA N6-methyladenosine reader IGF2BP3 regulates cell cycle and angiogenesis in colon cancer.; Authors - Zhou Yang
human	WB,IHC	Cell Death Dis; LncRNA CDKN2B-AS1 stabilized by IGF2BP3 drives the malignancy of renal clear cell carcinoma through epigenetically activating NUF2 transcription.; Authors - Xina Xie ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jessica (Verified Customer) (10-29-2018)

Also binds exogenous expressed IGF2BP1-GFP protein. I also get a double band around 70 kDa that is likely IGF2BP1 and IGF2BP3. This antibody is not specific.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: Neuroblastoma cells (human)

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