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IGF2BP3 Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 14642-1-AP

Featured Product KD/KO validated

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

IGF2BP3 Antibody WB HepG2 cells 14642-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 14642-1-AP(IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IGF2BP3 Antibody IHC human pancreas cancer tissue 14642-1-AP

Immunohistochemical of paraffin-embedded human pancreas cancer using 14642-1-AP(IGF2BP3 antibody) at dilution of 1:100 (under 40x lens)

IGF2BP3 Antibody IF MCF-7 cells 14642-1-AP

Immunofluorescent analysis of MCF-7 cells, using IGF2BP3 antibody 14642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

IGF2BP3 Antibody IP HeLa cells 14642-1-AP

IP Result of anti-IGF2BP3 (IP:14642-1-AP, 3ug; Detection:14642-1-AP 1:1000) with HeLa cells lysate 4400ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
HepG2 cells, BxPC-3 cells, HeLa cells, mouse brain tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human pancreas cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IGF2BP3 fusion protein Ag6226
Full name:
insulin-like growth factor 2 mRNA binding protein 3
Calculated molecular weight:
64 kDa
Observed molecular weight:
64 kDa
GenBank accession number:
BC065269
Gene ID (NCBI):
10643
Gene symbol
IGF2BP3
Synonyms
hKOC, IGF II mRNA binding protein 3, IGF2 mRNA binding protein 3, IGF2BP3, IMP 3, IMP3, KOC1, VICKZ family member 3, VICKZ3

Background

IGF2BP3, also named as IMP3, KOC1 and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

IGF2BP3 Rabbit Polyclonal antibody

Jessica Bell, 10-29-2018
Applications
Western Blot,
Primary Antibody Dilution
1:1500

Cell/Tissue Type
Neuroblastoma cells (human)
Lot Number
Also binds exogenous expressed IGF2BP1-GFP protein. I also get a double band around 70 kDa that is likely IGF2BP1 and IGF2BP3. This antibody is not specific.
Proteintech Response
Thank you for taking the time to review this antibody. Due to your feedback, we are going to put this product through our QC process again and, if it does not pass, we will re-examine our production process and make a new lot.

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