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  • KD/KO Validated

IGF2BP3 Polyclonal antibody

IGF2BP3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14642-1-AP

Synonyms

hKOC, IGF II mRNA binding protein 3, IGF2 mRNA binding protein 3, IGF2BP3, IMP 3, IMP3, KOC1, VICKZ family member 3, VICKZ3



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, mouse placenta tissue, BxPC-3 cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14642-1-AP targets IGF2BP3 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGF2BP3 fusion protein Ag6226
Full Name insulin-like growth factor 2 mRNA binding protein 3
Calculated molecular weight 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC065269
Gene symbol IGF2BP3
Gene ID (NCBI) 10643
RRIDAB_2122782
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.

Protocols

Product Specific Protocols
WB protocol for IGF2BP3 antibody 14642-1-APDownload protocol
IHC protocol for IGF2BP3 antibody 14642-1-APDownload protocol
IF protocol for IGF2BP3 antibody 14642-1-APDownload protocol
IP protocol for IGF2BP3 antibody 14642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

SIRT6 Suppresses Pancreatic Cancer through Control of Lin28b.

Authors - Sita Kugel
mouseWB

Cancer Cell

Lin28b is sufficient to drive liver cancer and necessary for its maintenance in murine models.

Authors - Liem H Nguyen
  • KD Validated
humanWB

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng
mouseRIP

Nat Cell Biol

N6-methyladenosine regulates maternal RNA maintenance in oocytes and timely RNA decay during mouse maternal-to-zygotic transition.

Authors - You Wu
humanWB

Mol Cell

m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3

Authors - Ting Shan
  • KD Validated
humanWB,RIP

Mol Cancer

Demethylase ALKBH5 suppresses invasion of gastric cancer via PKMYT1 m6A modification.

Authors - Yiyang Hu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

honglei (Verified Customer) (01-17-2023)

This antibody can be used for immunofluorescence and mainly localizes in the nucleus

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: cell line
FH

Jessica (Verified Customer) (10-29-2018)

Also binds exogenous expressed IGF2BP1-GFP protein. I also get a double band around 70 kDa that is likely IGF2BP1 and IGF2BP3. This antibody is not specific.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1500
  • Cell Tissue Type: Neuroblastoma cells (human)