|Positive WB detected in||HEK-293 cells, mouse placenta tissue, HeLa cells, HepG2 cells, BxPC-3 cells, mouse brain tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14642-1-AP targets IGF2BP3 in WB, RIP, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IGF2BP3 fusion protein Ag6226|
|Full Name||insulin-like growth factor 2 mRNA binding protein 3|
|Calculated molecular weight||64 kDa|
|Observed molecular weight||64 kDa|
|GenBank accession number||BC065269|
|Gene ID (NCBI)||10643|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IGF2BP3, also named as IMP3, KOC1 and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.
|Product Specific Protocols|
|WB protocol for IGF2BP3 antibody 14642-1-AP||Download protocol|
|IHC protocol for IGF2BP3 antibody 14642-1-AP||Download protocol|
|IF protocol for IGF2BP3 antibody 14642-1-AP||Download protocol|
|IP protocol for IGF2BP3 antibody 14642-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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EIF4G1 and RAN as Possible Drivers for Malignant Pleural Mesothelioma.
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RNA N6-methyladenosine reader IGF2BP3 regulates cell cycle and angiogenesis in colon cancer.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jessica (Verified Customer) (10-29-2018)
Also binds exogenous expressed IGF2BP1-GFP protein. I also get a double band around 70 kDa that is likely IGF2BP1 and IGF2BP3. This antibody is not specific.