|Positive WB detected in||A431 cells, L02 cells, HepG2 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human liver cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
20253-1-AP targets IGF2R-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||insulin-like growth factor 2 receptor|
|Calculated molecular weight||274 kDa|
|Observed molecular weight||300 kDa|
|GenBank accession number||NM_000876|
|Gene ID (NCBI)||3482|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.
|Product Specific Protocols|
|WB protocol for IGF2R-Specific antibody 20253-1-AP||Download protocol|
|IHC protocol for IGF2R-Specific antibody 20253-1-AP||Download protocol|
|IF protocol for IGF2R-Specific antibody 20253-1-AP||Download protocol|
|IP protocol for IGF2R-Specific antibody 20253-1-AP||Download protocol|
|FC protocol for IGF2R-Specific antibody 20253-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Agric Food Chem
Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.
Mol Biol Cell
Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.
Hum Mol Genet
Progranulin regulates lysosomal function and biogenesis through acidification of lysosomes.