IGF2R-Specific Polyclonal antibody

IGF2R-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20253-1-AP

Synonyms

CD222, CI Man 6 P receptor, CI MPR, CIMPR, IGF II receptor, IGF2R, M6P R, M6P/IGF2 receptor, M6P/IGF2R, M6PR, MPR 300, MPR1, MPRI



Tested Applications

Positive WB detected inA431 cells, L02 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

20253-1-AP targets IGF2R-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name insulin-like growth factor 2 receptor
Calculated molecular weight 274 kDa
Observed molecular weight 300 kDa
GenBank accession numberNM_000876
Gene symbol IGF2R
Gene ID (NCBI) 3482
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.

Protocols

Product Specific Protocols
WB protocol for IGF2R-Specific antibody 20253-1-APDownload protocol
IHC protocol for IGF2R-Specific antibody 20253-1-APDownload protocol
IF protocol for IGF2R-Specific antibody 20253-1-APDownload protocol
IP protocol for IGF2R-Specific antibody 20253-1-APDownload protocol
FC protocol for IGF2R-Specific antibody 20253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Agric Food Chem

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Authors - Shu Yang
humanWB, IF

Mol Biol Cell

Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.

Authors - Stephen C Ireland
humanWB

Hum Mol Genet

Progranulin regulates lysosomal function and biogenesis through acidification of lysosomes.

Authors - Yoshinori Tanaka