IGF2R-Specific Polyclonal antibody

IGF2R-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 20253-1-AP

Synonyms

IGF2R, CI MPR, CI Man-6-P receptor, CI Man 6 P receptor, CD222



Tested Applications

Positive WB detected inHepG2 cells, A431 cells, L02 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse colon tissue, human liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20253-1-AP targets IGF2R-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name insulin-like growth factor 2 receptor
Calculated Molecular Weight 274 kDa
Observed Molecular Weight 300 kDa
GenBank Accession NumberNM_000876
Gene Symbol IGF2R
Gene ID (NCBI) 3482
RRIDAB_10859779
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.

Protocols

Product Specific Protocols
WB protocol for IGF2R-Specific antibody 20253-1-APDownload protocol
IHC protocol for IGF2R-Specific antibody 20253-1-APDownload protocol
IF protocol for IGF2R-Specific antibody 20253-1-APDownload protocol
IP protocol for IGF2R-Specific antibody 20253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Progranulin regulates lysosomal function and biogenesis through acidification of lysosomes.

Authors - Yoshinori Tanaka
ratWB

J Agric Food Chem

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Authors - Shu Yang
humanWB, IF

Mol Biol Cell

Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.

Authors - Stephen C Ireland
humanWB

J Cancer

Trop2 binding IGF2R induces gefitinib resistance in NSCLC by remodeling the tumor microenvironment.

Authors - Xia Sun
humanWB

Life Sci Alliance

Identification and characterization of a membrane receptor that binds to human STC1.

Authors - Hin Ting Wan
ratWB,IHC

J Sci Food Agric

Grandmaternal betaine supplementation enhances hepatic IGF2 expression in F2 rat offspring through modification of promoter DNA methylation.

Authors - Shu Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Tiphaine (Verified Customer) (06-20-2022)

Localization seems to be coherent

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: HeLa cells
IGF2R-Specific Antibody Immunofluorescence validation (1/100 dilution) in HeLa cells (Cat no:20253-1-AP)
FH

Q (Verified Customer) (03-22-2021)

A reliable antibody to detect endogenous IGF2R with WB. Although there are a few non specific bands at lower levels.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain
IGF2R-Specific Antibody Western Blot validation (1:2000 dilution) in mouse brain (Cat no:20253-1-AP)