Tested Applications
| Positive WB detected in | HepG2 cells, A431 cells, L02 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse colon tissue, human liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20253-1-AP targets IGF2R-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | insulin-like growth factor 2 receptor |
| Calculated Molecular Weight | 274 kDa |
| Observed Molecular Weight | 300 kDa |
| GenBank Accession Number | NM_000876 |
| Gene Symbol | IGF2R |
| Gene ID (NCBI) | 3482 |
| RRID | AB_10859779 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11717 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
| IF protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
| IHC protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
| IP protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
| WB protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IGF2R-Specific Polyclonal antibody (Cat# 20253-1-AP) has 32 citations. Journals include Nature Biotechnology, Nature Communications, Journal of Extracellular Vesicles, Journal of Advanced Research, Metabolism, Redox Biology, Theranostics, Advanced Science, Cell Death & Disease, Journal of Cell Biology, mBio, Science Reports, iScience, Human Molecular Genetics, Molecular Biology of the Cell, and bioRxiv. Research fields span cancer (liver, lung, bladder, breast, cervical, pancreatic, laryngeal), diabetes, lysosome/Golgi trafficking, autophagy, neurodegeneration, viral infection, and epigenetics.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Salvianolic Acid B Upregulates miR-744-5p Expression in Peripheral Blood Mononuclear Cell-Derived Extracellular Vesicles to Alleviate Uremic Cardiomyopathy. | ||
Nat Commun GCAF(TMEM251) regulates lysosome biogenesis by activating the mannose-6-phosphate pathway | ||
J Adv Res CD34+CLDN5+ tumor associated senescent endothelial cells through IGF2-IGF2R signaling increased cholangiocellular phenotype in hepatocellular carcinoma | ||
Metabolism Nonenzymatic function of DPP4 promotes diabetes-associated cognitive dysfunction through IGF-2R/PKA/SP1/ERp29/IP3R2 pathway-mediated impairment of Treg function and M1 microglia polarization | ||
Redox Biol Sulfhydrated TFEB alleviates blast-induced lung injury by maintaining epithelial barrier integrity through the IGF2R/MMP-2/9 pathway. |
Reviews
What customers say
Both reviewers rated the antibody 4 out of 5 stars. One user applied it for immunofluorescence on HeLa cells at a 1/100 dilution and found the localization to be coherent. The other used it for Western Blot on mouse brain tissue at a 1:2000 dilution, describing it as a reliable antibody for detecting endogenous IGF2R, though noting a few non-specific bands at lower molecular weights. Overall, the antibody performs well in both IF and WB applications with minor non-specific binding observed in one case.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tiphaine (Verified Customer) (06-20-2022) | Localization seems to be coherent
 |
Q (Verified Customer) (03-22-2021) | A reliable antibody to detect endogenous IGF2R with WB. Although there are a few non specific bands at lower levels.
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