|Positive WB detected in||A431 cells, L02 cells, HepG2 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human liver cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
20253-1-AP targets IGF2R-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||bovine, human, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||insulin-like growth factor 2 receptor|
|Calculated molecular weight||274 kDa|
|Observed molecular weight||300 kDa|
|GenBank accession number||NM_000876|
|Gene ID (NCBI)||3482|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.
|Product Specific Protocols|
|WB protocol for IGF2R-Specific antibody 20253-1-AP||Download protocol|
|IHC protocol for IGF2R-Specific antibody 20253-1-AP||Download protocol|
|IF protocol for IGF2R-Specific antibody 20253-1-AP||Download protocol|
|IP protocol for IGF2R-Specific antibody 20253-1-AP||Download protocol|
|FC protocol for IGF2R-Specific antibody 20253-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Agric Food Chem
Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.
Rumen-Protected Glucose Stimulates the Insulin-Like Growth Factor System and mTOR/AKT Pathway in the Endometrium of Early Postpartum Dairy Cows.
Cell Death Dis
Autophagy-associated circular RNA hsa_circ_0007813 modulates human bladder cancer progression via hsa-miR-361-3p/IGF2R regulation.
J Sci Food Agric
Grandmaternal betaine supplementation enhances hepatic IGF2 expression in F2 rat offspring through modification of promoter DNA methylation.
Trop2 binding IGF2R induces gefitinib resistance in NSCLC by remodeling the tumor microenvironment.
Mol Biol Cell
Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Q (Verified Customer) (03-22-2021)
A reliable antibody to detect endogenous IGF2R with WB. Although there are a few non specific bands at lower levels.