Rat IL-1 beta Polyclonal antibody, PBS Only

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  WB analysis using 29530-1-AP (same clone as 29530-1-PBS)

  Untreated RAW 264.7 cells, RAW 264.7 cell (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for at least 3h) were subjected to SDS PAGE followed by western blot with 29530-1-AP (Il1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29530-1-PBS in a different storage buffer formulation.

IL-1 beta Polyclonal Antibody for WB, Indirect ELISA

Cat No. 29530-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, Indirect ELISA

Synonyms

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Product Information

29530-1-PBS targets IL-1 beta in WB, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: interleukin 1 beta
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: NM_031512
• Gene Symbol: IL-1 beta
• Gene ID (NCBI): 24494
• RRID: AB_2918322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q63264
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.