IL-1 beta Polyclonal antibody

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• IL-1 beta Polyclonal antibody (29530-1-AP)

IL-1 beta Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 29530-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

Il1 beta, Il1b, IL 1 beta, IL-1 beta,IL 1 beta,IL1beta, IL-1 β

• 

  WB analysis using 29530-1-AP

  Untreated RAW 264.7 cells, RAW 264.7 cell (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for at least 3h) were subjected to SDS PAGE followed by western blot with 29530-1-AP (Il1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of RAW 264.7 using 29530-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed LPS and Brefeldin A treated RAW 264.7 cells using IL-1 beta antibody (29530-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: LPS and Brefeldin A treated RAW 264.7 cells
• Positive IF/ICC detected in: LPS and Brefeldin A treated RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29530-1-AP targets IL-1 beta in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: interleukin 1 beta
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: NM_031512
• Gene Symbol: IL-1 beta
• Gene ID (NCBI): 24494
• RRID: AB_2918322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q63264
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Inflammation; ACE2 Alleviates Microglia Neuroinflammation by RANK-RANKL-OPG Axis in Parkinson's Disease; Authors - Yuxiang Xu
mouse	WB,ELISA	Front Immunol; Trueperella pyogenes promotes the synthesis and maturation of IL-1β in murine macrophages; Authors - Meimei Yang
mouse	WB	J Trace Elem Med Biol; The safety assessment of cinnabar: Effects of co-administration with selenium on renal toxicity in mice; Authors - Ziyan Han
mouse	WB	Ann Med; Astragaloside IV ameliorate acute alcohol-induced liver injury in mice via modulating gut microbiota and regulating NLRP3/caspase-1 signaling pathway; Authors - Shan Wu
mouse	WB	J Integr Med; Silk fibroin@polydopamine for acupoint catgut-embedding therapy in Alzheimer's disease.; Authors - Chun Wang
mouse	WB	iScience; The mechanisms and therapeutic potential of clopidogrel in mitigating diabetic cardiomyopathy in db/db mice; Authors - Bing Li