IL-1 beta Polyclonal antibody

• 

  WB analysis using 29530-1-AP

  Untreated RAW 264.7 cells, RAW 264.7 cell (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for at least 3h) were subjected to SDS PAGE followed by western blot with 29530-1-AP (Il1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IL-1 beta Polyclonal Antibody for WB, ELISA

Cat No. 29530-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, ELISA

Synonyms

IL 1 beta, IL-1 beta, Il1b, interleukin 1 beta, Interleukin-1 beta

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Tested Applications

• Positive WB detected in: LPS and Brefeldin A treated RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• ELISA: See 1 publications below

Product Information

29530-1-AP targets IL-1 beta in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: interleukin 1 beta
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: NM_031512
• Gene Symbol: IL-1 beta
• Gene ID (NCBI): 24494
• RRID: AB_2918322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q63264
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Front Pharmacol; Dapagliflozin promotes angiogenesis in hindlimb ischemia mice by inducing M2 macrophage polarization; Authors - Heng Yang
mouse	WB,ELISA	Front Immunol; Trueperella pyogenes promotes the synthesis and maturation of IL-1β in murine macrophages; Authors - Meimei Yang
mouse	WB	Ann Med; Astragaloside IV ameliorate acute alcohol-induced liver injury in mice via modulating gut microbiota and regulating NLRP3/caspase-1 signaling pathway; Authors - Shan Wu
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Farnesoid X receptor overexpression prevents hepatic steatosis through inhibiting AIM2 inflammasome activation in nonalcoholic fatty liver disease; Authors - Shuai Xu
rat	WB	Clin Nutr; Untargeted metabolomics and mendelian randomization analysis identify alpha-linolenic acid and linoleic acid as novel biomarkers of perioperative neurocognitive dysfunction; Authors - Xinxin Yang
mouse	WB	iScience; The mechanisms and therapeutic potential of clopidogrel in mitigating diabetic cardiomyopathy in db/db mice; Authors - Bing Li