Anti-Mouse IL-17A Rabbit Recombinant Antibody, PBS Only

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  FC experiment of C57BL/6 Th17-polarized splenocytes using 98005-1-PBS

  1x10^6 unstimulated or PMA and ionomycin stimulated (in the presence of protein transport inhibitors) Th17-polarized splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IL-17A (98005-1-PBS, Clone: 230038C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:700, and 0.06 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IL-17A Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98005-1-PBS

Clone No.230038C7

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

FC (Intra)

Synonyms

Il17a, IL-17A, interleukin 17A, Interleukin17A, 230038C7, IL 17A, Il17

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Tested Applications

• Positive FC (Intra) detected in: PMA and ionomycin stimulated C57BL/6 Th17-polarized splenocytes
• Positive FC detected in: PMA and ionomycin stimulated C57BL/6 Th17-polarized splenocytes

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
• Flow Cytometry (FC): FC :

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98005-1-PBS targets IL-17A in FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 17A
• Calculated Molecular Weight: 17 kDa
• GenBank Accession Number: NM-010552
• Gene Symbol: Il17a
• Gene ID (NCBI): 16171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q62386
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

IL-17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.