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NeutraKine® IL-23 p40 Monoclonal antibody

NeutraKine® IL-23 p40 Monoclonal Antibody for Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Neutralization

Conjugate

Unconjugated

CloneNo.

2A9H6

Cat no : 69006-1-Ig

Synonyms

IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL-23 p40, IL23A, IL-23A, IL23P19, Interleukin 23 subunit alpha, Interleukin 23 subunit p19, NeutraKine® IL-23 p40, SGRF

Validation Data Gallery

Applications:
  • Neutralization experiment of NeutraKine® IL-23 p40 using NeutraKine® IL-23 p40 Monoclonal antibody (69006-1-Ig)

    Neutralization experiment of NeutraKine® IL-23 p40 using 69006-1-Ig

    Recombinant human IL-23 (Cat.NO. HZ-1254) induces invitro cultured mouse splenocytes to secret IL-17A in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-23 (4 ng/mL HZ-1254) is neutralized by mouse anti-human IL-23 p40 monoclonal antibody 69006-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 8-30 ng/mL.

  • Neutralization experiment of NeutraKine® IL-23 p40 using NeutraKine® IL-23 p40 Monoclonal antibody (69006-1-Ig)

    Neutralization experiment of NeutraKine® IL-23 p40 using 69006-1-Ig

    Recombinant human IL-23 (Cat.NO. HZ-1254) induces in vitro cultured mouse splenocytes to secret IL-17A in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-23 (4 ng/mL HZ-1254) is neutralized by mouse anti-human IL-23 p40 monoclonal antibody 69006-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 8-30 ng/mL. The NeutraControl mouse anti-human IL-23 p40 monoclonal antibody 69506-1-Ig could recognize human IL-23, but could not neutralize human IL-23 in the range of 0.244-4000ng/mL(green curve, refer to top X-right Y axis).

  • ELISA experiment of Recombinant protein using NeutraKine® IL-23 p40 Monoclonal antibody (69006-1-Ig)

    ELISA experiment of Recombinant protein using 69006-1-Ig

    Indirect ELISA was carried out by coating recombinant Human IL-23 (Cat.NO. HZ-1254) at 70 ng/well followed by blocking and adding serial diluted IL-23 antibody 69006-1-Ig and 69506-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

Neutralization

Product Information

69006-1-Ig targets NeutraKine® IL-23 p40 in Neutralization applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human Humankine IL-23 p40 protein HZ-1254
Full Name interleukin 23, alpha subunit p19
Gene symbol IL23A
Gene ID (NCBI) 51561,3593
Conjugate Unconjugated
Form Lyophilized Powder
Purification Method Protein A purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Interleukin 23 (IL-23) is a member of the IL12 cytokine family and composed of two subunits, IL12p40 and IL23p19. It is produced by antigen presenting cells and has been shown to promote the production and survival of a distinct lineage of T-cells called TH17 cells. A functional receptor for IL-23 (the IL-23 receptor) has been identified and is composed of IL-12Rβ1 and IL-23R. IL-23 is expressed chiefly by the macrophages and DCs. The IL-23R is found on memory T cells, NKT cells, macrophages, DCs, and naive T cells upon activation by TGF-βand IL-6. The main biological effects of IL-23 identified initially are stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). IL-23 acts also as an end-stage effector cytokine through direct action on macrophages (PMID: 16393998;15657292;12023369;12610626).

This antibody can be used to neutralize the bioactivity of IL23 p40.