NeutraKine™ IL-6 Antibody

NeutraKine™ IL-6 Monoclonal Antibody for Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Neutralization, ELISA

Conjugate

Unconjugated

CloneNo.

4G3E6

Cat no : 69001-1-Ig

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, Interferon beta 2, Interleukin 6



Tested Applications

NeutralizationMeasured by its ability to neutralize IL-6 induced proliferation of hybridoma. The half neutralization dose (ND50) is typically 3-15 ng/mL in the presence of 1 ng/mL human IL-6.

Product Information

69001-1-Ig targets NeutraKine™ IL-6 in Neutralization, ELISA applications and shows reactivity with Human samples.

<0.1 EU/μg
Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human Humankine IL-6 protein HZ-1019
Full Name interleukin 6 (interferon, beta 2)
Calculated molecular weight
Observed molecular weight
GenBank accession number
Gene symbol IL6
Gene ID (NCBI) 3569
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer Sterile PBS.
ReconsitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

This antibody is used to Block/Neutralize IL6 protein.

Protocols

Product Specific Protocols
Neutralization protocol of NeutraKine™ IL-6 antibody 69001-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols