Validation Data Gallery
Technical Specifications
| GeneID | 3569 |
| Species | Human |
| Expression | HEK293 |
| Activity(Primary) | 0.03-0.24 ng/mL in 3G12B10 cells. |
| Activity(Secondary) | Typically <0.5 ng/mL EC50 in 7TD1 cells. |
| Specific Activity | minimally 3.00 x 107 IU/mg |
| Purity | >95% |
| Endotoxin | <1 EU/µg |
| Accession Number | P05231 |
| Molecular Mass | 24 and 29 kDa reduced, 21 and 28 kDa non-reduced, monomer, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human,mouse |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages. IL-6 plays an essential role in the final differentiation of B-cells into immunoglobulin-secreting cells. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity (PMID: 29736207; 29731693).
Synonyms
B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6
Publications
| Species | Title |
|---|---|
Small Methods Pre-Induced ICD Membrane-Coated Carrier-Free Nanoparticles for the Personalized Lung Cancer Immunotherapy | |
Cell Rep Obesity Drives Th17 Cell Differentiation by Inducing the Lipid Metabolic Kinase, ACC1. | |
Mol Metab IL-6/JAK2-dependent G6PD phosphorylation promotes nucleotide synthesis and supports tumor growth | |
Front Immunol Dendritic cell phenotype and function in a 3D co-culture model of patient-derived metastatic colorectal cancer organoids | |
Cancers (Basel) Silibinin Suppresses Tumor Cell-Intrinsic Resistance to Nintedanib and Enhances Its Clinical Activity in Lung Cancer. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Srinivas (Verified Customer) (06-17-2024) | The product is pure high reactive, I have used for check the human mast cell line to check the inflamtory response control when compare with IL-33 treatment in Metabolomics test
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