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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

Animal-free Recombinant Human IL-6

Activity

Typically ≤ 0.5 ng/mL EC50

Species Reactivity

Human

Purity

>95%

Publications

20

Cat no : HZ-1019

Synonyms

26 kDa protein, IFN-β2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2 IFNβ2, IFN β2, BSF2, BSF 2, MGI2, MGI 2



Technical Specifications

GeneID 3569
Species Human
Expression HEK293
Activity Typically ≤ 0.5 ng/mL EC50
Purity >95%
Molecular Mass 21 and 29 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human, mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Animal-free Recombinant Human IL-6 is produced in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 21 to 29 kDa. This broad molecular mass is attributable to glycosylation, which is absent when this cytokine is expressed in E. coli. Glycosylation contributes to stability in cell growth media and other applications. IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells, and keratinocytes, IL-6 has diverse biological functions.

Background

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages. IL-6 plays an essential role in the final differentiation of B-cells into immunoglobulin-secreting cells. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity (PMID: 29736207; 29731693).

Synonyms

26 kDa protein, IFN-β2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2 IFNβ2, IFN β2, BSF2, BSF 2, MGI2, MGI 2

Publications

SpeciesTitle
human

Breast Cancer (Dove Med Press)

Interleukin-6 is a potential therapeutic target in interleukin-6 dependent, estrogen receptor-α-positive breast cancer.

Authors - TinekeCasneuf
human

J Toxicol Sci

Human induced pluripotent stem cell-derived mast cells useful for in vitro mast cell activation assay exhibiting phenotypes and morphological characteristics of human mast cells.

Authors - Tatsuya Ikuno
llamas

Protein Eng Des Sel

Combining somatic mutations present in different in vivo affinity-matured antibodies isolated from immunized Lama glama yields ultra-potent antibody therapeutics.

Authors - AlexKlarenbeek
human

Biomed Microdevices

Study of melatonin-mediated effects on various hepatic inflammatory responses stimulated by IL-6 in a new HepG2-on-a-chip platform.

Authors - Mi Jang
human

Cancer Biomark

Interleukin-6 and oncostatin M are elevated in liver disease in conjunction with candidate hepatocellular carcinoma biomarker GP73.

Authors - HongyanLiang
human

Br J Clin Pharmacol

Development and validation of panoptic Meso scale discovery assay to quantify total systemic interleukin-6.

Authors - ShaliniChaturvedi