- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human IL-6 protein
Cat no : HZ-1019
Synonyms
B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6
Validation Data Gallery
Technical Specifications
| GeneID | 3569 |
| Species | human |
| Expression | HEK293 |
| Activity | Typically ≤ 0.5 ng/mL EC50 |
| Purity | >95% |
| Endotoxin | <1 EU/µg |
| Accession Number | P05231 |
| Molecular Mass | 21 and 29 kDa, monomer, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human,mouse |
Stability and Reconstitution
| Stability and Storage | Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products. |
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
Background
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages. IL-6 plays an essential role in the final differentiation of B-cells into immunoglobulin-secreting cells. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity (PMID: 29736207; 29731693).
Synonyms
B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6
Publications
| Species | Title |
|---|---|
Cell Death Discov Interleukin-6 derived from cancer-associated fibroblasts attenuates the p53 response to doxorubicin in prostate cancer cells. | |
J Immunol Res Downregulation of RIPK4 Expression Inhibits Epithelial-Mesenchymal Transition in Ovarian Cancer through IL-6. | |
ACS Biomater Sci Eng Bioprinting of Human Cord Blood-Derived CD34+ Cells and Exploration of the Multilineage Differentiation Ability in Vitro. | |
Breast Cancer (Dove Med Press) Interleukin-6 is a potential therapeutic target in interleukin-6 dependent, estrogen receptor-α-positive breast cancer. | |
J Toxicol Sci Human induced pluripotent stem cell-derived mast cells useful for in vitro mast cell activation assay exhibiting phenotypes and morphological characteristics of human mast cells. | |
Protein Eng Des Sel Combining somatic mutations present in different in vivo affinity-matured antibodies isolated from immunized Lama glama yields ultra-potent antibody therapeutics. |