IL-1 beta Antibody

IL-1 beta Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A1B4

Cat no : 66737-1-Ig

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta



Tested Applications

Positive WB detected inA431 cells, U-87 MG cells
Positive IHC detected inhuman lung cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66737-1-Ig targets IL-1 beta in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-1 beta fusion protein Ag26030
Full Name interleukin 1, beta
Calculated molecular weight 269aa,31 kDa
Observed molecular weight 35 kDa
GenBank accession number BC008678
Gene symbol IL1B
Gene ID (NCBI) 3553
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.

Protocols

Product Specific Protocols
WB protocol for IL-1 beta antibody 66737-1-IgDownload protocol
IHC protocol for IL-1 beta antibody 66737-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

BMJ Open Diabetes Res Care

KGF-2 and FGF-21 poloxamer 407 hydrogel coordinates inflammation and proliferation homeostasis to enhance wound repair of scalded skin in diabetic rats.

Authors - Xuanxin Yang
ratWB,IF

Neuroscience

Age-Associated Dopaminergic Neuron Loss and Midbrain Glia Cell Phenotypic Polarization.

Authors - GuoQing Wang
mouseWB

Neuroscience

Neuroprotective Action of Teriflunomide in a Mouse Model of Transient Middle Cerebral Artery Occlusion.

Authors - Zhengfang Lu
mouseIF

Neuropharmacology

Glimepiride and glibenclamide have comparable efficacy in treating acute ischemic stroke in mice.

Authors - Xiaoqiang Wang
humanIF

Biomaterials

Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo.

Authors - Angelina D Schoenenberger