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IL1 beta Antibody 3 Publications

Mouse Monoclonal| Catalog number: 66737-1-Ig |CloneNo.: 2A1B4

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

IL1 beta Antibody WB A431 cells 66737-1-Ig

A431 cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

IL1 beta Antibody IHC human lung cancer tissue 66737-1-Ig

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IL1 beta Antibody IHC human lung cancer tissue 66737-1-Ig

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
Human
Positive WB detected in:
A431 cells
Positive IHC detected in:
human lung cancer tissue, human skin cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IL1 beta fusion protein Ag26030
Full name:
interleukin 1, beta
Calculated molecular weight:
269aa,31 kDa
Observed molecular weight:
35 kDa
GenBank accession number:
BC008678
Gene ID (NCBI):
3553
Gene symbol
IL1B
Synonyms
Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

Background

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.


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