IL-1 Beta Monoclonal antibody

• Featured Product
• KD/KO Validated

IL-1 Beta Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IP, IHC, IF, FC, Cell treatment, ELISA

Conjugate

Unconjugated

Publications

55

CloneNo.

2A1B4

Cat no : 66737-1-Ig

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

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Validation Data Gallery

• 

  WB analysis of A431 using 66737-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-87 MG using 66737-1-Ig

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66737-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66737-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66737-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 66737-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 66737-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 66737-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens).

• 

  FC experiment of THP-1 using 66737-1-Ig

  1X10^6 THP-1 cells untreated (dashed lines), LPS treated (red) were intracellularly stained with 0.4 ug Anti-Human IL-1 Beta (66737-1-Ig, Clone:2A1B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.4 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, U-87 MG cells
• Positive IHC detected in: human lung cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: LPS treated THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 44 publications below
• IHC: See 8 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Product Information

66737-1-Ig targets IL-1 Beta in WB, IP, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, bovine
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-1 Beta fusion protein Ag26030
• Full Name: interleukin 1, beta
• Calculated molecular weight: 269 aa, 31 kDa
• Observed molecular weight: 35 kDa
• GenBank accession number: BC008678
• Gene symbol: IL1B
• Gene ID (NCBI): 3553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.

Publications

Species	Application	Title
human	IF	Biomaterials; Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo.; Authors - Angelina D Schoenenberger
mouse	IHC	J Nanobiotechnology; Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression; Authors - Yunhao Wang
human	WB	Emerg Microbes Infect; Acquiring high expression of suilysin enable non-epidemic Streptococccus suis to cause Streptococcal toxic shock-like syndrome (STSLS) through NLRP3 inflammasome hyperactivation.; Authors - Lei Xu
human	IHC	Thyroid; A MULTICENTER, SINGLE-BLIND, CASE-CONTROL, IMMUNOHISTOCHEMICAL STUDY OF ORBITAL TISSUE IN THYROID EYE DISEASE; Authors - YuanPing Hai
mouse	WB, IP	Front Immunol; The E3 Ubiquitin Ligase TRIM65 Negatively Regulates Inflammasome Activation Through Promoting Ubiquitination of NLRP3.; Authors - Tiantian Tang
human	IHC, WB	Int J Biol Sci; Cisplatin Induces Pyroptosis via Activation of MEG3/NLRP3/caspase-1/GSDMD Pathway in Triple-Negative Breast Cancer.; Authors - Honglin Yan