IL-1 beta Antibody

IL-1 beta Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

2A1B4

Cat no : 66737-1-Ig

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

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Validation Data Gallery

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  WB analysis of A431 cells using 66737-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

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  IHC staining of human lung cancer tissue using 66737-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

• 

  IHC staining of human lung cancer tissue using 66737-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

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Tested Applications

• Positive WB detected in: A431 cells, U-87 MG cells
• Positive IHC detected in: human lung cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IF: See 3 publications below

Product Information

66737-1-Ig targets IL-1 beta in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-1 beta fusion protein Ag26030
• Full Name: interleukin 1, beta
• Calculated molecular weight: 269aa,31 kDa
• Observed molecular weight: 35 kDa
• GenBank accession number: BC008678
• Gene symbol: IL1B
• Gene ID (NCBI): 3553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.

Publications

Species	Application	Title
rat	WB	BMJ Open Diabetes Res Care; KGF-2 and FGF-21 poloxamer 407 hydrogel coordinates inflammation and proliferation homeostasis to enhance wound repair of scalded skin in diabetic rats.; Authors - Xuanxin Yang
rat	WB,IF	Neuroscience; Age-Associated Dopaminergic Neuron Loss and Midbrain Glia Cell Phenotypic Polarization.; Authors - GuoQing Wang
mouse	WB	Neuroscience; Neuroprotective Action of Teriflunomide in a Mouse Model of Transient Middle Cerebral Artery Occlusion.; Authors - Zhengfang Lu
mouse	IF	Neuropharmacology; Glimepiride and glibenclamide have comparable efficacy in treating acute ischemic stroke in mice.; Authors - Xiaoqiang Wang
human	IF	Biomaterials; Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo.; Authors - Angelina D Schoenenberger