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- KD/KO Validated
IL-1 Beta Monoclonal antibody
IL-1 Beta Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Human and More (2)
WB, IP, IHC, IF, FC, Cell treatment, ELISA
Cat no : 66737-1-Ig
Validation Data Gallery
|Positive WB detected in||A431 cells, U-87 MG cells|
|Positive IHC detected in||human lung cancer tissue, human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||LPS treated THP-1 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 44 publications below|
|IHC||See 8 publications below|
|IF||See 6 publications below|
|IP||See 1 publications below|
|ELISA||See 1 publications below|
66737-1-Ig targets IL-1 Beta in WB, IP, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, bovine|
|Host / Isotype||Mouse / IgG1|
|Immunogen||IL-1 Beta fusion protein Ag26030|
|Full Name||interleukin 1, beta|
|Calculated molecular weight||269 aa, 31 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC008678|
|Gene ID (NCBI)||3553|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.
|Product Specific Protocols|
|WB protocol for IL-1 Beta antibody 66737-1-Ig||Download protocol|
|IHC protocol for IL-1 Beta antibody 66737-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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