IL-19 Monoclonal antibody

IL-19 Monoclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

8H10H8

Cat no : 60278-1-Ig

Synonyms

IL19, interleukin19, interleukin 19, IL 19, IL 10C

---

Validation Data Gallery

• 

  WB analysis of Recombinant protein using 60278-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60278-1-Ig (IL-19 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 60278-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60278-1-Ig (IL-19 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human tonsillitis using 60278-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-19 antibody (60278-1-Ig, Clone: 8H10H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 60278-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-19 antibody (60278-1-Ig, Clone: 8H10H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of U-937 using 60278-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U-937 cells using IL-19 antibody (60278-1-Ig, Clone: 8H10H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive IHC detected in: human tonsillitis tissue, human breast cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue
• Positive IF/ICC detected in: U-937 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60278-1-Ig targets IL-19 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-19 fusion protein Ag18887
• Full Name: interleukin 19
• Calculated Molecular Weight: 177 aa, 20 kDa
• GenBank Accession Number: BC153127
• Gene Symbol: IL-19
• Gene ID (NCBI): 29949
• RRID: AB_2881397
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-19, a member of the IL-10 cytokine family, is expressed in epithelial cells, endothelial cells, and macrophages. It can bind the interleukin-20 receptor complex IL-20R1/IL-20R2 and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). IL-19 induces IL-6 and TNF-alpha production in monocytes. It also induces cell apoptosis and reactive oxygen species production in monocytes, which suggests a role of this cytokine in inflammatory responses. It is up-regulated in monocytes following stimulation with lipopolysaccharide (LPS), and granulocyte-macrophage colony-stimulating factor (GM-CSF). IL-19 alters the balance of Th1 and Th2 cells in favor of Th2 cells. IL-19 contributes to a range of diseases and disorders, such as breast cancer, squamous cell carcinoma of the skin, asthma, endotoxic shock, uremia, psoriasis, rheumatoid arthritis, and periodontal and vascular disease.