IL-19 Monoclonal antibody

IL-19 Monoclonal Antibody for IF, IHC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

8H10H8

Cat no : 60278-1-Ig

Synonyms

IL 10C, IL 19, IL19, IL-19, interleukin 19, MDA1, NG.1, ZMDA1



Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

60278-1-Ig targets IL-19 in IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-19 fusion protein Ag18887
Full Name interleukin 19
Calculated molecular weight 177 aa, 20 kDa
GenBank accession numberBC153127
Gene symbol IL19
Gene ID (NCBI) 29949
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL-19, a member of the IL-10 cytokine family, is expressed in epithelial cells, endothelial cells, and macrophages. It can bind the interleukin-20 receptor complex IL-20R1/IL-20R2 and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). IL-19 induces IL-6 and TNF-alpha production in monocytes. It also induces cell apoptosis and reactive oxygen species production in monocytes, which suggests a role of this cytokine in inflammatory responses. It is up-regulated in monocytes following stimulation with lipopolysaccharide (LPS), and granulocyte-macrophage colony-stimulating factor (GM-CSF). IL-19 alters the balance of Th1 and Th2 cells in favor of Th2 cells. IL-19 contributes to a range of diseases and disorders, such as breast cancer, squamous cell carcinoma of the skin, asthma, endotoxic shock, uremia, psoriasis, rheumatoid arthritis, and periodontal and vascular disease.

Protocols

Product Specific Protocols
WB protocol for IL-19 antibody 60278-1-IgDownload protocol
IHC protocol for IL-19 antibody 60278-1-IgDownload protocol
IF protocol for IL-19 antibody 60278-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols