CoraLite®594-conjugated IL-19 Monoclonal antibody

IL-19 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

8H10H8

Cat no : CL594-60278

Synonyms

IL 10C, IL 19, IL19, IL-19, interleukin 19, MDA1, NG.1, ZMDA1

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL594-60278

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 IL-19 antibody (CL594-60278, Clone: 8H10H8 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-60278

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 IL-19 antibody (CL594-60278, Clone: 8H10H8 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60278 targets IL-19 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-19 fusion protein Ag18887
• Full Name: interleukin 19
• Calculated Molecular Weight: 177 aa, 20 kDa
• GenBank Accession Number: BC153127
• Gene Symbol: IL19
• Gene ID (NCBI): 29949
• RRID: AB_2919910
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-19, a member of the IL-10 cytokine family, is expressed in epithelial cells, endothelial cells, and macrophages. It can bind the interleukin-20 receptor complex IL-20R1/IL-20R2 and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). IL-19 induces IL-6 and TNF-alpha production in monocytes. It also induces cell apoptosis and reactive oxygen species production in monocytes, which suggests a role of this cytokine in inflammatory responses. It is up-regulated in monocytes following stimulation with lipopolysaccharide (LPS), and granulocyte-macrophage colony-stimulating factor (GM-CSF). IL-19 alters the balance of Th1 and Th2 cells in favor of Th2 cells. IL-19 contributes to a range of diseases and disorders, such as breast cancer, squamous cell carcinoma of the skin, asthma, endotoxic shock, uremia, psoriasis, rheumatoid arthritis, and periodontal and vascular disease.