IL-21R Polyclonal antibody

IL-21R Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10533-1-AP

Synonyms

CD360, IL 21 receptor, IL 21R, IL21R, IL-21R, interleukin 21 receptor, NILR, Novel interleukin receptor

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Validation Data Gallery

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  WB analysis using 10533-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10533-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10533-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 10533-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10533-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 10533-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10533-1-AP (IL21R Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 10533-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10533-1-AP (IL21R Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of COLO 320 using 10533-1-AP

  Immunofluorescent analysis of COLO 320 cells using 10533-1-AP (IL21R antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of K-562 using 10533-1-AP

  1X10^6 K-562 cells were stained with 0.2ug IL21R antibody (10533-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: COLO 320 cells, K-562 cells, mouse thymus tissue, Raji cells, rat thymus tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: COLO 320 cells
• Positive FC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

10533-1-AP targets IL-21R in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-21R fusion protein Ag0762
• Full Name: interleukin 21 receptor
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC004348
• Gene Symbol: IL21R
• Gene ID (NCBI): 50615
• RRID: AB_2124107
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL21R, a receptor for interleukin-21, belongs to the type I cytokine receptor family. IL21R has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3.

Publications

Species	Application	Title
human	IHC	J Immunother Cancer; Prognostic value of novel immune-related genomic biomarkers identified in head and neck squamous cell carcinoma.; Authors - Yao Yao
human	WB,IF	Stem Cells Int; Interleukin 21 Receptor Affects Adipogenesis of Human Adipose-Derived Stem/Stromal Cells.; Authors - Bruna Cristina Falavinha
rat	WB,IF	Int Immunopharmacol; Role of Interleukin-21 in retinal ischemia-reperfusion injury: Unveiling the impact on retinal ganglion cell apoptosis; Authors - Xue Zhang