|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
16545-1-AP targets IL-4 in WB, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|IL-4 fusion protein Ag9871
|Calculated molecular weight
|153 aa, 17 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
What is the molecular weight of IL-4?
IL-4 has a calculated weight of 14.9 kDa.
What cell type produces the IL-4 protein?
Mast cells in peripheral tissues produce the cytokines IL-4 and IL-13, both of which regulate response to environmental allergens. IL-4 in particular is synthesized rapidly following T cell activation to then stimulate downstream anti-inflammatory processes.
What activity does IL-4 stimulate?
IL-4 is a highly polyfunctional cytokine, contributing to the regulation of many cellular and immunological processes. Increases in IL-4 levels stimulate IL-13 production as well as helper T cell and B cell proliferative responses to anti-immunoglobulin antibodies. IL-4 also aids in controlling T helper cell type 2 (Th2) differentiation, driving differentiation of naive T cells to the Th2 pathway.
How do IL-4 levels respond to brain damage?
Ischemic neurons may secrete IL-4 to help decrease proinflammatory activity of macrophages.(PMID: 26269636)
How do IL-4 levels respond to allergens?
Asthmatics express stimulated IL-4 bronchoalveolar lavage during allergen challenges.
Can polymorphisms in the Il4 gene lead to asthma?
Polymorphisms resulting in mutant IL-4 proteins, are associated with asthma risk, development, and severity.
What does the IL-4: IFN-g ratio signify?
IL-4 promotes Th2 differentiation and stability, promoting its own production while inhibiting Th1 cell differentiation. The Th1 pathway, however, produces interferon gamma (IFN-g). The IL-4:IFN-g ratio gives indication as to which pathway is being enhanced or depressed.
PMIDs: 26088754, 26255210, 25819429,10556256
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