• Featured Product
  • KD/KO Validated

IL-4 Monoclonal antibody

IL-4 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G2C5

Cat no : 66142-1-Ig

Synonyms

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, PS1TP5BP1



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66142-1-Ig is IL-4 Fusion Protein expressed in E. coli.

Tested Reactivity human, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-4 fusion protein Ag9940
Full Name interleukin 4
Calculated molecular weight 153 aa, 17 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC070123
Gene symbol IL4
Gene ID (NCBI) 3565
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.

Protocols

Product Specific Protocols
WB protocol for IL-4 antibody 66142-1-IgDownload protocol
IHC protocol for IL-4 antibody 66142-1-IgDownload protocol
IF protocol for IL-4 antibody 66142-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Transl Stroke Res

Beneficial Effects of Theta-Burst Transcranial Magnetic Stimulation on Stroke Injury via Improving Neuronal Microenvironment and Mitochondrial Integrity.

Authors - Xuemei Zong
humanIHC

Thyroid

A MULTICENTER, SINGLE-BLIND, CASE-CONTROL, IMMUNOHISTOCHEMICAL STUDY OF ORBITAL TISSUE IN THYROID EYE DISEASE

Authors - YuanPing Hai
ratWB

Front Cell Dev Biol

DJ-1 Regulates Microglial Polarization Through P62-Mediated TRAF6/IRF5 Signaling in Cerebral Ischemia-Reperfusion.

Authors - Tingting Wang
ratELISA

Exp Neurol

Photobiomodulation therapy promotes neurogenesis by improving post-stroke local microenvironment and stimulating neuroprogenitor cells.

Authors - Luodan Yang
mouseWB

Mol Neurobiol

Quercetin Alleviates Demyelination Through Regulating Microglial Phenotype Transformation to Mitigate Neuropsychiatric Symptoms in Mice with Vascular Dementia.

Authors - Zihu Tan
humanWB,IF

Food Funct

Chitooligosaccharides alleviate hepatic fibrosis by regulating the polarization of M1 and M2 macrophages.

Authors - Peng Liu