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- KD/KO Validated
IL-4 Monoclonal antibody
IL-4 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 66142-1-Ig
Validation Data Gallery
|Positive WB detected in||Jurkat cells|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human breast cancer tissue|
|Positive FC detected in||Ramos cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66142-1-Ig targets IL-4 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig samples.
|Tested Reactivity||human, pig|
|Cited Reactivity||human, mouse, rat, pig, bovine|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||IL-4 fusion protein Ag9940|
|Full Name||interleukin 4|
|Calculated molecular weight||153 aa, 17 kDa|
|Observed molecular weight||18 kDa|
|GenBank accession number||BC070123|
|Gene ID (NCBI)||3565|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.
|Product Specific Protocols|
|WB protocol for IL-4 antibody 66142-1-Ig||Download protocol|
|IHC protocol for IL-4 antibody 66142-1-Ig||Download protocol|
|IF protocol for IL-4 antibody 66142-1-Ig||Download protocol|
|FC protocol for IL-4 antibody 66142-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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