CoraLite®594-conjugated IL-4 Monoclonal antibody

IL-4 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G2C5

Cat no : CL594-66142

Synonyms

IL4, interleukin 4, IL 4, BSF-1, B-cell stimulatory factor 1

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Validation Data Gallery

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  IF Staining of Raji using CL594-66142

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CoraLite®594 IL-4 antibody (CL594-66142, Clone: 1G2C5 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66142 targets IL-4 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-4 fusion protein Ag9940
• Full Name: interleukin 4
• Calculated Molecular Weight: 153 aa, 17 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC070123
• Gene Symbol: IL-4
• Gene ID (NCBI): 3565
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.