COLO 320 cells were subjected to SDS PAGE followed by western blot with 10179-1-AP( Mitofilin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10179-1-AP(Mitofilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10179-1-AP(Mitofilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HepG2 cells were subjected to SDS PAGE followed by western blot with 10179-1-AP( Mitofilin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

human brain tissue were subjected to SDS PAGE followed by western blot with 10179-1-AP(Mitofilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

MCF7 cells were subjected to SDS PAGE followed by western blot with 10179-1-AP(Mitofilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

WB result of 10179-1-AP(Mitofilin antibody) at dliution of 1:1000.

Raji cells were subjected to SDS PAGE followed by western blot with 10179-1-AP(Mitofilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunohistochemical of paraffin-embedded human breast cancer using 10179-1-AP(Mitofilin antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human breast cancer using 10179-1-AP(Mitofilin antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical of paraffin-embedded human gliomas using 10179-1-AP(Mitofilin antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human gliomas using 10179-1-AP(Mitofilin antibody) at dilution of 1:100 (under 40x lens)

IP Result of anti-Mitofilin (IP:10179-1-AP, 3ug; Detection:10179-1-AP 1:1000) with Raji cells lysate 3800ug.

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 10179-1-AP(Mitofilin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of HepG2 cells, using IMMT antibody 10179-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

1X10^6 HEK-293T cells were stained with .2ug Mitofilin antibody (10179-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.