Mitofilin Polyclonal antibody

• Featured Product
• KD/KO Validated

Mitofilin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

34

Reviews (3)

Cat no : 10179-1-AP

Synonyms

HMP, IMMT, MIC60, MINOS2, Mitofilin, P87, P87/89, P89, PIG4, PIG52

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Validation Data Gallery

• 

  WB analysis using 10179-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10179-1-AP (Mitofilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 10179-1-AP

  IP Result of anti-Mitofilin (IP:10179-1-AP, 3ug; Detection:10179-1-AP 1:1000) with Raji cells lysate 3800ug.

• 

  IHC staining of human gliomas using 10179-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10179-1-AP (Mitofilin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human gliomas using 10179-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10179-1-AP (Mitofilin antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 10179-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10179-1-AP (Mitofilin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293T using 10179-1-AP

  1X10^6 HEK-293T cells were stained with .2ug Mitofilin antibody (10179-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: COLO 320 cells, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, mouse brain tissue
• Positive IP detected in: Raji cells
• Positive IHC detected in: human gliomas tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, HepG2 cells
• Positive FC detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 8 publications below
• WB: See 32 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Product Information

10179-1-AP targets Mitofilin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: hamster, human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Mitofilin fusion protein Ag0102
• Full Name: inner membrane protein, mitochondrial (mitofilin)
• Calculated molecular weight: 90 kDa
• Observed molecular weight: 80-90 kDa
• GenBank accession number: BC002412
• Gene symbol: IMMT
• Gene ID (NCBI): 10989
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.

Publications

Species	Application	Title
human	WB	PLoS Genet; TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.; Authors - Peng Wang
human		Mol Cell; ER and Nutrient Stress Promote Assembly of Respiratory Chain Supercomplexes through the PERK-eIF2α Axis.; Authors - Eduardo Balsa ; KO Validated
mouse	WB	mSphere; Toxoplasma gondii MAF1b Binds the Host Cell MIB Complex To Mediate Mitochondrial Association.; Authors - Felice D Kelly ; KD Validated
human	WB	Cell Death Dis; OPA1 and MICOS Regulate mitochondrial crista dynamics and formation.; Authors - Chao Hu ; KO Validated
rat	WB,IF,CoIP	Am J Transl Res; Parkin interacts with Mitofilin to increase dopaminergic neuron death in response to Parkinson's disease-related stressors.; Authors - Abdulhafiz D Imam Aliagan ; KD Validated
human	WB	Biochemistry; Pink1 forms a multiprotein complex with Miro and Milton, linking Pink1 function to mitochondrial trafficking.; Authors - Weihofen Andreas A

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

CYNTHIA (Verified Customer) (07-16-2020)

I used the mitofilin on my WB samples at the beginning it was great but in 3 times of using it i couldn't understand why it doesn't work. but i recommend ure antibodies to my colleagues

• Applications: Western Blot

FH

SITING (Verified Customer) (07-13-2020)

SHOW VERY STRONG SIGNAL

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MOUSE HEART

FH

Yan (Verified Customer) (01-28-2020)

This antibody worked very well on mouse heart tissue for western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

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