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Mitofilin Polyclonal antibody

Mitofilin Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 10179-1-AP


HMP, IMMT, MIC60, MINOS2, Mitofilin, P87, P87/89, P89, PIG4, PIG52

Tested Applications

Positive WB detected inCOLO 320 cells, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, mouse brain tissue, MCF-7 cells, rat brain tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHEK-293T cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10179-1-AP targets Mitofilin in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Mitofilin fusion protein Ag0102
Full Name inner membrane protein, mitochondrial (mitofilin)
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC002412
Gene Symbol IMMT
Gene ID (NCBI) 10989
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.


Product Specific Protocols
WB protocol for Mitofilin antibody 10179-1-APDownload protocol
IHC protocol for Mitofilin antibody 10179-1-APDownload protocol
IF protocol for Mitofilin antibody 10179-1-APDownload protocol
IP protocol for Mitofilin antibody 10179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Methods

Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models.

Authors - Mark Bates

Cell Metab

Liver mitochondrial cristae organizing protein MIC19 promotes energy expenditure and pedestrian locomotion by altering nucleotide metabolism

Authors - Jee Hyung Sohn

Cell Metab

A cold-stress-inducible PERK/OGT axis controls TOM70-assisted mitochondrial protein import and cristae formation.

Authors - Pedro Latorre-Muro

Cell Metab

The Ca(2+)-Dependent Release of the Mia40-Induced MICU1-MICU2 Dimer from MCU Regulates Mitochondrial Ca(2+) Uptake.

Authors - Carmelina Petrungaro

Mol Cell

Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis

Authors - Yi Fu

Mol Cell

The interplay between BAX and BAK tunes apoptotic pore growth to control mitochondrial-DNA-mediated inflammation.

Authors - Katia Cosentino


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Mia (Verified Customer) (09-28-2022)

very clear band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mitochondria protein

siiting (Verified Customer) (03-20-2022)

very good antibody, second time buy already

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse tissue

Maria (Verified Customer) (08-15-2021)

Mitofilin in human primary fibroblasts. Blocking with 5% milk in PBST (0.1%t tween) 1h RT. Primary ab 1:1000 in BSA 3% in PBST O/N at 4º incubation. Secondary 1:5000 HRP 1h RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human primary fibroblasts
Mitofilin Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:10179-1-AP)

CYNTHIA (Verified Customer) (07-16-2020)

I used the mitofilin on my WB samples at the beginning it was great but in 3 times of using it i couldn't understand why it doesn't work. but i recommend ure antibodies to my colleagues

  • Applications: Western Blot

SITING (Verified Customer) (07-13-2020)


  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MOUSE HEART

Yan (Verified Customer) (01-28-2020)

This antibody worked very well on mouse heart tissue for western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue